Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression

Scientific Reports
Antonio José Ruiz-AlcarazMaría Martínez-Esparza

Abstract

Peritoneal macrophages play a critical role in the control of infectious and inflammatory diseases. Although recent progress on murine peritoneal macrophages has revealed multiple aspects on their origin and mechanisms involved in their maintenance in this compartment, little is known on the characteristics of human peritoneal macrophages in homeostasis. Here, we have studied by flow cytometry several features of human peritoneal macrophages obtained from the peritoneal cavity of healthy women. Three peritoneal monocyte/macrophage subsets were established on the basis of CD14/CD16 expression (CD14++CD16-, CD14++CD16+ and CD14highCD16high), and analysis of CD11b, CD11c, CD40, CD62L, CD64, CD80, CD86, CD116, CD119, CD206, HLA-DR and Slan was carried out in each subpopulation. Intracellular expression of GATA6 and cytokines (pro-inflammatory IL-6 and TNF-α, anti-inflammatory IL-10) as well as their phagocytic/oxidative activities were also analyzed, in an attempt to identify genuine resident peritoneal macrophages. Results showed that human peritoneal macrophages are heterogeneous regarding their phenotype, cell complexity and functional abilities. A direct relationship of CD14/CD16 expression, intracellular content of GATA6, and ...Continue Reading

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Citations

Nov 12, 2019·Immunology and Cell Biology·Antonio J Ruiz-AlcarazMaría Martínez-Esparza
Sep 20, 2020·European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [et Al] = Zeitschrift Für Kinderchirurgie·Nagoud SchukfehGertrud Vieten
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Feb 6, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christian E NiehausMarkus Cornberg
Oct 7, 2020·Journal for Immunotherapy of Cancer·Maria ZagorulyaStefani Spranger
Apr 20, 2021·Frontiers in Immunology·Arjuna Singanayagam, Evangelos Triantafyllou
May 28, 2021·Journal of Hepatology·Schalk Van der MerweAgustin Albillos
Jun 1, 2021·Frontiers in Immunology·Simone N ZwickyJoel Zindel
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Oct 7, 2021·Journal of Leukocyte Biology·Jasmine J M ChuahNicole K Campbell

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Datasets Mentioned

BETA
GM-CSFR

Methods Mentioned

BETA
FACS
flow cytometry
PMA
Fluorescence
lavage
FCS

Software Mentioned

Flowing
Cytomics RXP Analysis
SPSS
GraphPad Prism
FACS Canto

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