Characterization of human Sec16B: indications of specialized, non-redundant functions.

Scientific Reports
Annika BudnikDavid J Stephens

Abstract

The endoplasmic reticulum (ER) represents the entry point into the secretory pathway and from here newly synthesized proteins and lipids are delivered to the Golgi. The selective cargo export from the ER is mediated by COPII-assembly at specific sites of the ER, the so-called transitional ER (tER). The peripheral membrane protein Sec16, first identified in yeast, localizes to transitional ER and plays a key role in organization of these sites. Sec16 defines the tER and is thought to act as a scaffold for the COPII coat assembly. In humans two isoforms of Sec16 are present, the larger Sec16A and the smaller Sec16B. Nevertheless, the functional differences between the two isoforms are ill-defined. Here we describe characterization of the localization and dynamics of Sec16B relative to Sec16A, provide evidence that Sec16B is likely a minor or perhaps specialized form of Sec16, and that it is not functionally redundant with Sec16A.

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L OrciR Schekman
Oct 1, 1995·The Journal of Cell Biology·P EspenshadeC A Kaiser
Oct 1, 1996·The Journal of Cell Biology·S I BannykhW E Balch
Nov 1, 1996·Molecular Biology of the Cell·R E GimenoC A Kaiser
Nov 5, 1997·The Journal of Biological Chemistry·D A ShaywitzC A Kaiser
May 16, 2000·Life Sciences·M Yamaguchi
Sep 12, 2000·Molecular Biology of the Cell·A T Hammond, B S Glick
Sep 18, 2002·The Journal of Cell Biology·Frantisek SupekRandy Schekman
Mar 17, 2005·Cellular and Molecular Life Sciences : CMLS·M P Mayer, B Bukau
Aug 23, 2005·Current Biology : CB·Pamela L ConnerlyBenjamin S Glick
Jan 25, 2006·Current Biology : CB·Rebecca ForsterRainer Pepperkok
Apr 13, 2007·The Journal of Biological Chemistry·Takayuki IinumaKatsuko Tani
Jul 11, 2008·Molecular Biology of the Cell·Viorica IvanCatherine Rabouille
Aug 30, 2008·Biochimica Et Biophysica Acta·Glenn E Morris
Oct 22, 2008·The Journal of Cell Biology·Laura Trinkle-MulcahyAngus Lamond
Feb 14, 2009·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi
Apr 24, 2009·Molecular Biology of the Cell·Krysten J PalmerDavid J Stephens
Jul 30, 2009·Journal of Cell Science·Helen HughesDavid J Stephens
Oct 24, 2009·FEBS Letters·Annika Budnik, David J Stephens
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Valérie Lallemand-Breitenbach, Hugues de Thé
Aug 11, 2010·The Journal of Cell Biology·James R R Whittle, Thomas U Schwartz
Apr 12, 2011·Nature Cell Biology·Kristen WitteAnjon Audhya

❮ Previous
Next ❯

Citations

Sep 6, 2013·Molecular Biology of the Cell·Nike BharuchaBenjamin S Glick
Jan 27, 2015·Biochemical Society Transactions·Joep Sprangers, Catherine Rabouille
Aug 6, 2016·Nature Communications·Ilka WilhelmiFlorian Heyd

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB063357

Methods Mentioned

BETA
confocal
electron microscopy
transfection
PCR
immunoprecipitation
one hybrid
FCS
fluorescence recovery after photobleaching
transfections

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.