Characterization of human serum gamma-glutamyltranspeptidase

Clinica Chimica Acta; International Journal of Clinical Chemistry
A TsujiN Katunuma

Abstract

The contribution of proteolysis to leakage of gamma-glutamyltranspeptidase from the liver into the serum was examined by comparing the properties of human serum gamma-glutamyltranspeptidase with those of partially purified enzyme preparations solubilized from human liver with Triton X-100 and with papain. The Triton-solubilized enzyme was hydrophobic, whereas the papain-solubilized enzyme was hydrophilic. Serum gamma-glutamyltranspeptidase was found to be hydrophilic, since it could be extracted without addition of detergent after acetone treatment to remove bound cholate. The affinity of serum-gamma-glutamyltranspeptidase to concanavalin A-Sepharose was compared with those of the Triton-solubilized and papain-solubilized enzymes. All three enzymes were adsorbed to concanavalin A-Sepharose in 50 mmol/l imidazole buffer, pH 7.2. The serum enzyme and papain-solubilized enzyme were eluted with 100 mmol/l alpha-methylmannoside in 50 mmol/l imidazole buffer containing 1% Triton X-100. The molecular weights of the Triton-solubilized, papain-solubilized and serum-gamma-glutamyltranspeptidases were estimated as 98 000 +/- 2000, 90 000 +/- 2000 and 90 000 +/- 2000, respectively, by electrophoresis. These results show chemical similariti...Continue Reading

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S S Tate, A Meister
Jul 8, 1977·Biochimica Et Biophysica Acta·N E Huseby
Feb 10, 1978·Biochimica Et Biophysica Acta·N E Huseby
Jul 3, 1978·European Journal of Biochemistry·S HoriuchiY Morino
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Nov 1, 1966·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Szewczuk
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P RiegertJ Kaufman
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

Feb 1, 1989·Bulletin of Environmental Contamination and Toxicology·Y ShuklaN K Mehrotra
Apr 27, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·P R WenhamA F Smith
Sep 1, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·N E Huseby
Dec 29, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·D RatanasavanhG Siest
Mar 15, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·A R SajjanK S Datta
Jan 1, 1983·Advances in Enzyme Regulation·Y MatsudaN Katunuma
Apr 1, 1997·Toxicology and Applied Pharmacology·Y TakahashiM Joyce-Brady
Apr 5, 2005·Toxicologic Pathology·Philip F Solter
Jan 1, 1992·Renal Failure·J Pedraza-ChaverriJ C Peña
May 15, 1986·Archives of Biochemistry and Biophysics·J L ConeP P Roller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved