PMID: 9181552Apr 1, 1997Paper

Characterization of human type II procollagen and collagen-specific antibodies and their application to the study of human type II collagen processing and ultrastructure

Matrix Biology : Journal of the International Society for Matrix Biology
S A JimenezGeorge R Dodge

Abstract

Type II collagen is the most abundant collagen in articular cartilage and, together with other tissue-specific collagens and proteoglycans, provides the tissue with its shock-absorbing properties and its resiliency to stress. Specific antibodies which recognize various collagen types have been very useful in the study of collagen biosynthesis, structure and metabolism in normal and pathological conditions. Antibodies which recognize epitopes of type II collagen have been described previously; however, many of these antibodies display cross-reactivity with other collagens or with type II collagen from other species, reflecting the high degree of homology of the helical domains of fibrillar collagens. In this study, we prepared antibodies to sequential determinants of human type II procollagen employing synthetic peptides with sequences deduced from the nucleotide sequence of the human alpha 1 (II) procollagen cDNA. The antibodies were highly specific for epitopes in either the C-terminal propeptide or the telopeptide of the human type II collagen and did not cross-react with other human interstitial collagens or with murine type II collagen. These antibodies were used in conjunction with biosynthetic labeling to study the secret...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1991·Matrix : Collagen and Related Research·G R DodgeA R Poole
May 1, 1973·European Journal of Biochemistry·P Dehm, D J Prockop
Jan 15, 1981·Analytical Biochemistry·P Bruckner, D J Prockop
Nov 17, 1980·FEBS Letters·M ShimokomakiA J Bailey
Aug 1, 1994·Microscopy Research and Technique·P Bruckner, M van der Rest

❮ Previous
Next ❯

Citations

Sep 11, 2014·Journal of Biomedical Materials Research. Part a·Stacey C SkaalureStephanie J Bryant
Jan 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P U RaniJ S Khillan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.