Characterization of humic substances by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Samuel M Mugo, Christina S Bottaro

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and laser desorption/ionization (LDI-)TOFMS have been used to characterize Suwannee River humic substances, obtained from the International Humic Substances Society (IHSS), and Armadale soil fulvic acid (ASFA). An array of MALDI matrices were tested for use with humic substances, including alpha-cyano-4-hydroxycinammic acid (CHCA), 2-(4-hydroxyphenylazo)benzoic acid (HABA), 2,5-dihydroxybenzoic acid (DHBA), sinapinic acid, dithranol and norharmane. DHBA yielded the best results, exhibiting superior ionization efficiency, low noise, broad applicability to the analytes of interest, and most importantly producing an abundance of high mass ions, the highest observed being m/z 1848. A number of sample preparation modes were investigated; the overlayer method improved sample/matrix homogeneity and hence shot-to-shot reproducibility. The choice of the matrix, mass ratio of analyte to matrix, and the sample preparation protocol, were found to be the most critical factors governing the quality of the mass spectra. Matrix suppression was greatly enhanced by ensuring good mixing of matrix and analyte in the solid phase, proper optimization of the ma...Continue Reading

References

May 10, 2001·Rapid Communications in Mass Spectrometry : RCM·G PlancqueC Moulin
Feb 6, 2003·Journal of Chromatography. a·Pavel Janos
Feb 13, 2003·Chemical Reviews·Michael Karas, Ralf Krüger
Sep 1, 1995·Environmental Science & Technology·F J NovotnyD A Weil

❮ Previous
Next ❯

Citations

Feb 6, 2007·Journal of Chromatography. a·Benito CañasJose Manuel Gallardo
Apr 19, 2008·Rapid Communications in Mass Spectrometry : RCM·Gabriela ChilomJames A Rice
Jan 28, 2014·Journal of Mass Spectrometry : JMS·Ren-Qi WangJean-Philippe Croué
Mar 24, 2017·Analytical Chemistry·John W T BlackburnDušan Uhrín

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.