PMID: 9188598Jul 1, 1997Paper

Characterization of humoral and CD4+ cellular responses after genetic immunization with retroviral vectors expressing different forms of the hepatitis B virus core and e antigens

Journal of Virology
Matti SällbergDavid R Milich

Abstract

The humoral and CD4+ cellular immune responses in mice following genetic immunization with three retroviral vectors encoding different forms of hepatitis B virus core antigen (HBcAg) and e antigen (HBeAg) were analyzed. The retroviral vectors induced expression of intracellular HBcAg (HBc[3A4]), secreted HBeAg (HBe[5A2]), or an intracellular HBcAg-neomycin phosphoryltransferase fusion protein (HBc-NEO[6A3]). Specific antibody levels and immunoglobulin G isotype restriction were highly dependent on both the host major histocompatibility complex and the transferred gene. Humoral and CD4+ cellular HBcAg and/or HBeAg (HBc/eAg)-specific immune responses following retroviral vector immunization were of a lower magnitude but followed the same characteristics compared with those after immunization with HBc/eAg in adjuvant. Two factors influenced the humoral responses. First, in vivo depletion of CD8+ cells in HBc-NEO[6A3]-immunized H-2k mice abrogated both HBcAg-specific antibodies and in vitro-detectable cytotoxic T lymphocytes. Second, priming of H-2b mice with an HBc/eAg-derived T-helper (Th) peptide in adjuvant prior to retroviral vector immunization greatly enhanced the HBc/eAg-specific humoral responses to all three vectors, sugg...Continue Reading

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R MilichJ E Jones
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D R MilichA McLachlan
Oct 10, 1985·Nature·P TiollaisA Dejean
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J H OuW J Rutter
Feb 1, 1972·Cellular Immunology·R E ClickB J Alter
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·D R MilichJ E Jones
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L MichelR G Whalen
Jun 1, 1993·The Journal of Clinical Investigation·T MaruyamaD R Milich
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·E RazD A Carson

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Citations

May 15, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Hyung Joon KimByung Chul Yoo
May 9, 2000·Clinical and Diagnostic Laboratory Immunology·J IroegbuM Sällberg
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael J PalmowskiMary K Collins
Mar 17, 2015·Jundishapur Journal of Microbiology·Reza TaherkhaniAli Reza Samarbafzadeh
Jul 1, 1997·Human Gene Therapy·J E McCormackJ Warner
Jan 31, 2002·World Journal of Gastroenterology : WJG·Z H HuangW F Zhu
Mar 19, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Chandan GuhaJayanta Roy-Chowdhury
Dec 15, 2015·Expert Review of Vaccines·Margaret ChenMatti Sällberg

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