Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi.

Veterinary Research
Edita JeklováMartin Faldyna

Abstract

Encephalitozoonosis is a common infectious disease widely spread among rabbits. Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts. The aim of the study was to describe in detail the spread of the E. cuniculi in a rabbit organism after experimental infection and the host humoral and cellular immune response including cytokine production. For that purpose, healthy immunocompetent rabbits were infected orally in order to simulate the natural route of infection and euthanised at 2, 4, 6 and 8-weeks post-infection. Dissemination of E. cuniculi in the body of the rabbit was more rapid than previously reported. As early as 2 weeks post-infection, E. cuniculi was detected using immunohistochemistry not only in the intestine, mesenteric lymph nodes, spleen, liver, kidneys, lungs and heart, but also in nervous tissues, especially in medulla oblongata, cerebellum, and leptomeninges. Based on flow cytometry, no conspicuous changes in lymphocyte subpopulations were detected in the examined lymphoid organs of infected rabbits. Cell-mediated immunity was characterized by ability of both CD4+ and CD8+ T cells to proliferate after stimulation with sp...Continue Reading

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Citations

Mar 9, 2021·Parasite Immunology·Praisy AseejaSonali K Kalra

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Methods Mentioned

BETA
density gradient centrifugation
ELISA
flow cytometry
FCS
PCR

Software Mentioned

GraphPad
GraphPad Prism
GeNorm
blast
Primer
FACSDiva
BD

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