PMID: 7016533Apr 1, 1981Paper

Characterization of hydrolase H, a new muscle protease possessing aminoendopeptidase activity

European Journal of Biochemistry
Akihiro OkitaniH Kato

Abstract

A newly-identified protease from rabbit skeletal muscle, named hydrolase H [Okitani, A. et al. (1980) Agric. Biol. Chem. 44, 1705-1708] has been shown to hydrolyze L-leucine beta-naphthylamide as well as alpha-N-benzoyl-DL-arginine beta-naphthylamide. The enzyme hydrolyzes protamine in the manner of an endopeptidase and hydrolyzes tripeptides and tetrapeptides in the manner of an aminopeptidase. Thus it has been concluded that the enzyme possesses the properties of both endopeptidase and aminopeptidase and that it should be classified as an aminoendopeptidase. Both activities of endopeptidase and aminopeptidase are maximal at pH 7.5-8.0 and enhanced remarkably by thiol compounds. Both activities are stable at pH 7-9 and protected by 2-mercaptoethanol. They are inhibited by monoiodoacetic acid, leupeptin, Zn2+ and Ni2+, but not affected by EDTA, trypsin inhibitor, pepstatin, antipain and phenylmethylsulfonyl fluoride. These results suggest that the enzyme is a thiol protease. The enzyme is composed of three kinds of subunits, the chain Mr of which are 51000, 72000 and 92000.

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Citations

Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y MakinodanH Toyohara
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y MakinodanM Kinoshita
May 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T NishimuraH Kato
Feb 5, 2003·The International Journal of Biochemistry & Cell Biology·Masanori MatsuishiHiromichi Kato
Aug 15, 1992·European Journal of Biochemistry·M R RhyuH Kato
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Mohammed Alamgir SarkerToshihide Nishimura
Aug 5, 1985·FEBS Letters·D Kulesza-Lipka, A Jakubiec-Puka
Jan 26, 1983·Biochimica Et Biophysica Acta·F Ismail, W Gevers
Apr 1, 1987·The Journal of Investigative Dermatology·R J HarvimaW L Epstein
Nov 14, 1984·Biochemical and Biophysical Research Communications·J C Liao, J F Lenney

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