Characterization of hypovirus-derived small RNAs generated in the chestnut blight fungus by an inducible DCL-2-dependent pathway

Journal of Virology
Xuemin ZhangDonald L Nuss

Abstract

The disruption of one of two dicer genes, dcl-2, of the chestnut blight fungus Cryphonectria parasitica was recently shown to increase susceptibility to mycovirus infection (G. C. Segers, X. Zhang, F. Deng, Q. Sun, and D. L. Nuss, Proc. Natl. Acad. Sci. USA 104:12902-12906, 2007). We now report the accumulation of virus-derived small RNAs (vsRNAs) in hypovirus CHV1-EP713-infected wild-type and dicer gene dcl-1 mutant C. parasitica strains but not in hypovirus-infected dcl-2 mutant and dcl-1 dcl-2 double-mutant strains. The CHV1-EP713 vsRNAs were produced from both the positive and negative viral RNA strands at a ratio of 3:2 in a nonrandom distribution along the viral genome. We also show that C. parasitica responds to hypovirus and mycoreovirus infections with a significant increase (12- to 20-fold) in dcl-2 expression while the expression of dcl-1 is increased only modestly (2-fold). The expression of dcl-2 is further increased ( approximately 35-fold) following infection with a hypovirus CHV1-EP713 mutant that lacks the p29 suppressor of RNA silencing. The combined results demonstrate the biogenesis of mycovirus-derived small RNAs in a fungal host through the action of a specific dicer gene, dcl-2. They also reveal that dcl-...Continue Reading

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E V KooninJ C Carrington
Aug 26, 1999·Trends in Genetics : TIG·A Fire
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
May 23, 2002·Science·Phillip D Zamore
May 23, 2002·Science·Hongwei LiShou Wei Ding
Jul 19, 2003·Science·James C Carrington, Victor Ambros
Sep 16, 2003·Molecular Plant-microbe Interactions : MPMI·Naoki KadotaniShigeyuki Mayama
Mar 3, 2004·Molecular and Cellular Biology·Caterina CatalanottoCarlo Cogoni
May 1, 2004·Science·Sébastien PfefferThomas Tuschl
Jul 20, 2004·The Plant Cell·Byung-Chun YooWilliam J Lucas
Sep 17, 2004·Nature·David Baulcombe
Nov 6, 2004·Science·Binzhang Shen, Howard M Goodman
Nov 9, 2004·Advances in Virus Research·Bradley I Hillman, Nobuhiro Suzuki
Dec 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kimberly M KeeneKen E Olson
Feb 11, 2005·Nature Reviews. Genetics·Olivier Voinnet
Mar 3, 2005·Genes & Development·Yukihide Tomari, Phillip D Zamore
Mar 23, 2005·Nature Methods·Sébastien PfefferThomas Tuschl
Apr 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xuezhong CaiBryan R Cullen
Apr 22, 2005·Journal of Molecular Biology·Marita OverhoffIan Robbins
Apr 23, 2005·Science·Charles-Henri LecellierOlivier Voinnet
Apr 27, 2005·Cell·Edwards AllenJames C Carrington
May 28, 2005·Journal of Virology·Christopher S Sullivan, Don Ganem
Jun 9, 2005·Current Opinion in Plant Biology·Patrice Dunoyer, Olivier Voinnet
Aug 3, 2005·Nature Reviews. Microbiology·Donald L Nuss
Sep 6, 2005·Science·Phillip D Zamore, Benjamin Haley
Sep 6, 2005·Science·Catherine L JoplingPeter Sarnow
Sep 13, 2005·FEBS Letters·Hong-Wei Li, Shou-Wei Ding
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·H Alexander EbhardtPeter J Unrau
Dec 21, 2005·Virology·Sylvia Schütz, Peter Sarnow
Mar 24, 2006·Nature Immunology·Delphine Galiana-ArnouxJean-Luc Imler
May 13, 2006·Current Genetics·Heriberto Cerutti, J Armando Casas-Mollano
May 26, 2006·The EMBO Journal·Lóránt LakatosJózsef Burgyán

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Citations

Oct 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xuemin Zhang, Donald L Nuss
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xian-Bing WangShou-Wei Ding
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qihong SunDonald L Nuss
Jul 5, 2011·Eukaryotic Cell·Yunkun DangYi Liu
Apr 30, 2009·Annual Review of Phytopathology·Said A Ghabrial, Nobuhiro Suzuki
Apr 15, 2011·Uirusu·Sotaro ChibaNobuhiro Suzuki
Jul 28, 2013·International Journal of Molecular Sciences·Francisco E Nicolás, Rosa M Ruiz-Vázquez
Jul 4, 2012·Annual Review of Microbiology·Shwu-Shin ChangYi Liu
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Qingfa WuShou-Wei Ding
Aug 4, 2010·Cellular and Molecular Life Sciences : CMLS·Liande LiYi Liu
Mar 1, 2011·FEBS Letters·Xiaolin LiQun He
Jan 5, 2011·Protein & Cell·Thien HoHui Wang
Oct 29, 2008·Current Opinion in Microbiology·Hitoshi Nakayashiki, Quoc Bao Nguyen
Jan 6, 2009·Immunological Reviews·Roghiyh Aliyari, Shou-Wei Ding
Dec 7, 2014·The Journal of General Virology·Eeva J VainioJari P T Valkonen
Mar 17, 2015·Virology·Said A GhabrialNobuhiro Suzuki
Oct 20, 2015·Virus Research·Eeva J Vainio, Jarkko Hantula
May 14, 2016·Wiley Interdisciplinary Reviews. RNA·Eric Roberto Guimarães Rocha AguiarJoão Trindade Marques
Dec 17, 2014·PLoS Pathogens·Zhixiang ZhangQingfa Wu
Sep 1, 2016·Molecular Plant Pathology·Livia Donaire, María A Ayllón
Aug 14, 2010·Nature Reviews. Immunology·Shou-Wei Ding
Jan 11, 2019·Journal of Virology·Sabitree ShahiNobuhiro Suzuki

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