PMID: 7544233Jun 1, 1995Paper

Characterization of IgE-binding epitopes on Candida albicans enolase

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
K ItoS Torii

Abstract

Candida albicans enolase is one of the important allergens in Candida allergy. We isolated and purified 46kDa C. albicans enolase (CAE) from C. albicans and characterized epitopes for IgE antibody by lectin-blotting and enzymatic digestion followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Lectin blotting and deglycozilation indicated that this protein did not contain polysaccharide side chains. The purified CAE and recombinant fusion protein produced from CAE gene possessed common epitopes for IgE antibody. We estimated IgE binding epitopes on the basis of reported amino acid sequences from the analysis of cDNA encoding CAE. V8 protease digestion of CAE gave six polypeptide fragments (A-F). The N-termini of each fragment were confirmed by amino acid sequence and the C-termini were estimated by molecular weights of each fragment and the specific cutting site of V8 protease. Fragment C (25.0 kDa; F-171-I-399) reacted to 90% IgE antibodies examined, whereas fragments D (21.0 kDa; F-171-I-360), E (16.2 kDa; F-171-D-317) and F (13.0 kDa; A-47-E-170) showed no IgE binding. Our results suggest that epitopes for IgE antibodies exist near the C-terminal of the protein.

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Citations

Jul 29, 2005·Medical Microbiology and Immunology·Esperanza RodríguezTeresa Gárate
Aug 24, 2000·Allergy·E González-ManceboF León
Aug 22, 2007·International Archives of Allergy and Immunology·Birgit Simon-NobbeMichael Breitenbach
Jun 19, 2014·The World Allergy Organization Journal·Barbara CasesEnrique Fernandez-Caldas
Jan 20, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T KanbeA Ishiguro
May 1, 2007·Experimental Parasitology·Hak Sun YuHae Jin Jeong
Dec 17, 2014·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Brett J GreenDonald H Beezhold
Jan 12, 2010·Deutsches Ärzteblatt International·Jürgen Schulze, Ulrich Sonnenborn
Mar 3, 2021·PLoS Pathogens·Pedro Henrique Gazzinelli-GuimaraesThomas B Nutman
Feb 11, 1998·Clinical Microbiology Reviews·J P MartínezW L Chaffin

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