PMID: 2497076May 15, 1989Paper

Characterization of immunoglobulin mRNA expression in Burkitt lymphoma cell lines

International Journal of Cancer. Journal International Du Cancer
R AnkerB A Pollok

Abstract

Immunoglobulin heavy- and light-chain mRNA of 11 Burkitt lymphoma (BL) cell lines (9 African and 2 American) were analyzed for various structural characteristics. In agreement with previous results at the protein level, all the BL cell lines express heavy-chain mRNA transcripts of the mu class. Surprisingly, a high mu s/mu m mRNA ratio was found in 2 IgM-producing BL cell lines (Raji and CCL85), that do not secrete immunoglobulin. Variable region gene use was also assessed in the cell lines: while 4 out of 7 endemic BL cell lines use VH genes that belong to the VH3 gene family, no clear bias in the expression of particular VH or VL gene families among this sampling of BL lines was found. Northern blot analysis of immunoglobulin transcripts in endemic BL cell lines did show that 2 such lines (AG876 and HTB62) expressed truncated heavy-chain transcripts; RNA sequence analysis of the VH region demonstrated different abnormal 5'-localized RNA splicing events for the 2 shortened mu transcripts. The light-chain mRNA in these 2 cell lines also showed structural abnormalities and, in the case of HTB62, 3 different kappa light-chain transcripts are produced (of elongated, native and truncated sizes). In vitro translation of mRNA from HT...Continue Reading

References

Jan 1, 1979·Immunological Reviews·M SeligmannJ C Brouet
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A NeriR Dalla-Favera
Aug 15, 1988·International Journal of Cancer. Journal International Du Cancer·C D GregoryA B Rickinson
Apr 15, 1985·International Journal of Cancer. Journal International Du Cancer·M RoweM A Epstein
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A BakhshiS J Korsmeyer
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T J KippsD A Carson
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A ShenF Blattner
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B A PollokD Levitt
Oct 1, 1988·The Journal of Clinical Investigation·A AlexanderJ Buxbaum
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J GeliebterS G Nathenson
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·C M CroceY Tsujimoto
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A AlexanderJ N Buxbaum

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.