PMID: 9006022Feb 1, 1997Paper

Characterization of in-frame proteins encoded by cvaA, an essential gene in the colicin V secretion system: CvaA* stabilizes CvaA to enhance secretion

Journal of Bacteriology
J HwangP C Tai

Abstract

Colicin V (ColV), an antibacterial peptide toxin, uses a dedicated signal sequence-independent export system for its extracellular secretion in Escherichia coli. The products of at least three genes (a chromosomal tolC gene and two plasmid-born cvaA and cvaB genes) are involved in this process. To characterize the gene products, the cvaA gene was subcloned and expressed under the control of T7 RNA polymerase promoter. Two in-frame proteins, CvaA and CvaA*, were expressed and identified. DNA sequences predicted that both proteins have two potential translational initiation sites. N-terminal peptide sequencing showed that the translation of CvaA starts from a TTG, 11 amino acids upstream of the previously proposed ATG initiation site. CvaA* is translated from an upstream ATG. Expression of both CvaA and CvaA* was induced by the iron chelator 2,2'-dipyridyl, indicating that cvaA is negatively regulated at least partially by Fur. CvaA*-depleted cells were found to secrete less ColV, based on reduced activity in the supernatant, than did wild type, which was recovered by the addition of a plasmid producing CvaA*. Interestingly, CvaA*-depleted and wild-type cells had similar levels of intracellular ColV activity. Translational fusion...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D A Steege
Jun 25, 1975·Journal of Molecular Biology·J G FilesJ H Miller
Dec 1, 1975·Bacteriological Reviews·K G Hardy
Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C Wandersman, P Delepelaire
Jul 1, 1989·Molecular & General Genetics : MGG·M R Adhin, J van Duin
Jun 1, 1987·Journal of Bacteriology·L GilsonR Kolter
Jan 1, 1985·Journal of Bacteriology·P L Worsham, J Konisky
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jul 1, 1985·Journal of Bacteriology·T FelmleeR A Welch
Jul 1, 1985·European Journal of Biochemistry·G WeyensA Piérard
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Jun 1, 1966·Journal of Molecular Biology·B F Clark, K A Marcker
Sep 1, 1967·Journal of Bacteriology·H R Herschman, D R Helinski
Nov 1, 1981·Journal of Bacteriology·K K FrickJ Konisky
Jan 1, 1983·Journal of Cellular Biochemistry·M HärtleinW Goebel
May 1, 1984·Journal of Bacteriology·C C Yang, J Konisky
Jul 5, 1981·Journal of Molecular Biology·C NapoliB S Singer
Nov 1, 1995·Journal of Bacteriology·R C SkvirskyX Shen
Jan 1, 1993·Trends in Biochemical Sciences·M D Marger, M H Saier

❮ Previous
Next ❯

Citations

Dec 4, 2001·FEMS Microbiology Letters·M VarcamontiJ Kok
Jan 15, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Van Thi LeSittichai Koontongkaew
May 10, 2020·FEMS Microbiology Reviews·Daria FijalkowskaPetra Van Damme
Jul 14, 2018·Microbiology Spectrum·Sezen MeydanAlexander S Mankin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.