Characterization of in vitro metabolites of trimethoprim and diaveridine in pig liver microsomes by liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry

Biomedical Chromatography : BMC
Zhao-Ying LiuLeren Wan


Trimethoprim (TMP) and diaveridine (DVD) are used in combination with sulfonamides and sulfaquinoxlaine as an effective antibacterial agent and antiprotozoal agent, respectively, in humans and animals. To gain a better understanding of the metabolism of TMP and DVD in the food-producing animals, the metabolites incubated with liver microsomes of pigs were analyzed for the first time with high-performance liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry. Seven TMP-related and six DVD-related metabolites were characterized based on the accurate MS² spectra and known structure of the parent drug, respectively. The metabolites of TMP were identified as two O-demethylation metabolites, a di-O-demethylation metabolite, two N-oxides metabolites, a hydroxylated metabolite on the methylene carbon and a hydroxylated metabolite on the methyl group. DVD was also biotransformed to two O-demethylation metabolites, a di-O-demethylation metabolite, an N-oxide metabolite, a hydroxylation metabolite on the methylene carbon and a hydroxylation metabolite followed by O-demethylation. The results indicate that the two compounds have similar biotransformation pathways in pigs. O-Demethylation was the major metabol...Continue Reading


Jan 1, 1994·Research in Veterinary Science·P Nielsen, N Gyrd-Hansen
Jul 16, 1999·Mayo Clinic Proceedings·J D Smilack
Jan 29, 2002·Environmental and Molecular Mutagenesis·Tomoko Ono-OgataHideo Yamagata
Sep 26, 2008·Chemical Research in Toxicology·Micaela C DamstenJan N M Commandeur
Sep 1, 1997·Environmental Toxicology and Pharmacology·T OnoT Ohta

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