Characterization of In Vitro Reconstructed Human Normotrophic, Hypertrophic, and Keloid Scar Models

Tissue Engineering. Part C, Methods
G C LimandjajaS Gibbs

Abstract

To understand scar pathology, develop new drugs, and provide a platform for personalized medicine, physiologically relevant human scar models are required, which are characteristic of different scar pathologies. Hypertrophic scars and keloids are two types of abnormal scar resulting from unknown abnormalities in the wound healing process. While they display different clinical behavior, differentiation between the two can be difficult-which in turn means that it is difficult to develop optimal therapeutic strategies. The aim of this study was to develop in vitro reconstructed human hypertrophic and keloid scar models and compare these to normotrophic scar and normal skin models to identify distinguishing biomarkers. Keratinocytes and fibroblasts from normal skin and scar types (normotrophic, hypertrophic, keloid) were used to reconstruct skin models. All skin models showed a reconstructed differentiated epidermis on a fibroblast populated collagen-elastin matrix. Both abnormal scar types showed increased contraction, dermal thickness, and myofibroblast staining compared to normal skin and normotrophic scar. Notably, the expression of extracellular matrix associated genes showed distinguishing profiles between all scar types and ...Continue Reading

References

Jul 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L R AdkisonJ Gardner
Mar 12, 1998·Molecular Medicine Today·T L Tuan, L S Nichter
Feb 24, 2001·The Journal of Burn Care & Rehabilitation·R TsouN S Gibran
Jul 27, 2002·Plastic and Reconstructive Surgery·Thomas A MustoeUNKNOWN International Advisory Panel on Scar Management
Aug 13, 2003·Artificial Organs·Fábio KamamotoMarcus Castro Ferreira
Sep 6, 2003·The Journal of Surgical Research·Wei ChenZhiyong Sheng
May 19, 2004·Archives of Facial Plastic Surgery·Behrooz A TorkianBrian J F Wong
Jul 1, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Abdoelwaheb El Ghalbzouri, Maria Ponec
Sep 15, 2004·The American Journal of Dermatopathology·Julia Yu-Yun LeeTak-Wah Wong
Jan 19, 2005·The Journal of Investigative Dermatology·Abdoelwaheb El GhalbzouriMaria Ponec
Jan 19, 2005·The Journal of Investigative Dermatology·Aziz GhaharyMichael Weinfeld
Mar 18, 2005·The Journal of Pathology·Judith BellemareVéronique J Moulin
May 19, 2005·Annals of Plastic Surgery·Bishara S AtiyehShady N Hayek
Aug 30, 2005·Lasers in Surgery and Medicine·Lynn L ChiuBrian J F Wong
Dec 6, 2005·Plastic and Reconstructive Surgery·Andrew Burd, Lin Huang
Mar 15, 2006·Cells, Tissues, Organs·Hai S DuongDiana V Messadi
Jan 1, 1996·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·H SatoK Yoshizato
Jan 1, 1993·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K SaharaM Robson
Feb 6, 2007·Clinics in Dermatology·David T Robles, Daniel Berg
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sander W SpiekstraSusan Gibbs
Dec 12, 2007·American Journal of Surgery·Paris D ButlerGeorge P Yang
Feb 3, 2009·Cell Transplantation·Abraham P VriensSusan Gibbs
Apr 11, 2009·Archives of Dermatological Research·Oliver Seifert, Ulrich Mrowietz
Sep 23, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Pauline D H M VerhaegenEsther Middelkoop
Dec 17, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Barbara ShihArdeshir Bayat
Dec 15, 2010·The Journal of Trauma·Anandaroop MukhopadhyayThang T Phan
Jul 13, 2011·Journal of Burn Care & Research : Official Publication of the American Burn Association·Kevin L McFarlandDorothy M Supp
Aug 16, 2011·Journal of the European Academy of Dermatology and Venereology : JEADV·G P Sidgwick, A Bayat
Nov 2, 2011·Burns : Journal of the International Society for Burn Injuries·Franck SimonVéronique J Moulin
Apr 19, 2012·Experimental Dermatology·Rania BagabirArdeshir Bayat
Jul 13, 2012·The American Journal of Dermatopathology·Chen HuangXia Zhao
May 1, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jie DingEdward E Tredget
Apr 8, 2015·Photodermatology, Photoimmunology & Photomedicine·Jenifer Mendoza-GarciaArdeshir Bayat
May 6, 2015·Experimental Dermatology·Lenie J van den BroekFrank B Niessen
Dec 20, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Yun-Shain LeeTai-Lan Tuan
Jun 7, 2016·Drug Discovery Today·Lambert I J C BergersSusan Gibbs
Jul 6, 2016·The British Journal of Dermatology·G C LimandjajaS Gibbs

❮ Previous
Next ❯

Citations

Jan 5, 2019·PloS One·Bruno Grosselli LaniaPaulo Eduardo Neves Ferreira Velho
Jun 13, 2019·Archives of Dermatological Research·Grace C LimandjajaSusan Gibbs
Jun 13, 2019·Journal of Burn Care & Research : Official Publication of the American Burn Association·Diana ForbesReza Jalili
Oct 30, 2018·Archives of Dermatological Research·Grace C LimandjajaSusan Gibbs
Jun 13, 2020·Frontiers in Cell and Developmental Biology·Grace C LimandjajaSusan Gibbs
Mar 2, 2021·Lasers in Surgery and Medicine·Jinxia ZhangRongya Yang
Jun 10, 2021·Burns : Journal of the International Society for Burn Injuries·Jinxia ZhangRongya Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
enzyme-linked immunosorbent assays
PCR
ELISA
biopsies
scar

Software Mentioned

NIS
elements
Elements AR
GraphPad Prism

Related Concepts

Related Feeds

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.