Characterization of In Vivo Reporter Systems for Gene Expression and Biosensor Applications Based on luxAB Luciferase Genes.

Applied and Environmental Microbiology
K BlouinR Turner

Abstract

Advances in genetic engineering methods have allowed the development of an increasing number of practical and scientific applications for bioluminescence with lux genes cloned from a variety of organisms. Bioluminescence derived from the shortened lux operon (luxAB genes) is a complex process, and applications seem to be proliferating in advance of an understanding of the underlying biochemical processes. In this report, we describe a two-phase kinetic behavior of the light emission which must be properly taken into account in any quantitative measurements of the bioluminescence signal. By using strains of Escherichia coli and Caulobacter crescentus, this behavior was characterized and interpreted in terms of the biochemistry underlying the bacterial luciferase mechanism. We show that the intensity profile of each of the two phases of the luminescence signal is responsive (and exhibits different sensitivities) to the concentration of added decanal and other components of the assay mix, as well as to the order of mixing and incubation times. This study illustrates the importance of appropriate protocol design, and specific recommendations for using the luxAB system as a molecular reporter are presented, along with versatile assa...Continue Reading

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Citations

May 22, 2007·Applied and Environmental Microbiology·Herwig BachmannJohan E T van Hylckama Vlieg
Aug 9, 2008·Journal of Environmental Monitoring : JEM·Ademola O OlaniranBalakrishna Pillay
Nov 7, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Janne AtosuoEsa-Matti Lilius
May 27, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Myu YoshidaKaneyoshi Yamamoto
Jun 29, 2002·Applied and Environmental Microbiology·R R YarwoodP J Bottomley
Apr 18, 2003·Luminescence : the Journal of Biological and Chemical Luminescence·Gérald ThouandMichel Rousseau
May 24, 2011·Analytical and Bioanalytical Chemistry·Aldo RodaMarko Virta
Nov 1, 2017·FEMS Microbiology Letters·Tomohiro ShimadaAkira Ishihama
Jan 10, 2019·FEMS Microbiology Letters·Yukari MiyakeAkira Ishihama
Feb 7, 2002·Journal of Microbiological Methods·Angela KeanePeter C K Lau
Oct 8, 2021·Critical Reviews in Biotechnology·Jian ZhangQian Wang

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