Characterization of IncC Plasmids in Enterobacterales of Food-Producing Animals Originating From China

Frontiers in Microbiology
Yu ZhangHong-Ning Wang

Abstract

Incompatibility group C (IncC) plasmids have received attention due to their broad host range and because they harbor key antibiotic resistance genes. Because these resistance genes can spread from food-producing animals to human, the proliferation of these plasmids represents a public health risk. In this study, a total of 20 IncC plasmids were collected from food-producing animals in China, and characterized by Oxford Nanopore Technologies long-read sequencing. Based on four key differences of the IncC backbone, 4 IncC plasmids were classified as type 1, 15 were classified as type 1/2 hybrid, and one was classified as type 2. The 15 type 1/2 hybrids were further divided into 13 type 1/2a and 2 type 1/2b, based on sequence differences arising from different homologous recombination events between type 1 and type 2 IncC backbones. Genome comparison of accessory resistance modules showed that different IncC plasmids exhibited various phenotypes via loss and gain of diverse modules, mainly within the bla CMY -carrying region, and two antibiotic resistance islands designated ARI-A and ARI-B. Interestingly, in addition to insertion and deletion events, IS26 or IS1294-mediated large sequence inversions were found in the IncC genome...Continue Reading

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Citations

Jul 5, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·João Pedro Rueda FurlanEliana Guedes Stehling

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Datasets Mentioned

BETA
JQ010984
MT560001
CP041629

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

SPAdes
Blast
MLST
Unicycler GPLv3
PlasmidFinder
NanoPlot
IntegronFinder Galaxy
RAST
Easyfig
Rapid Annotation using Subsystem Technology ( RAST )

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