Characterization of inducible stx2-positive Escherichia coli O157:H7/H7- strains isolated from cattle in France

Journal of Applied Microbiology
Claire MaurerJ-Y Madec

Abstract

To quantify the variability of the Shiga toxin 2 (Stx2) production by a panel of stx2-positive Escherichia coli O157:H7/H7- isolates from healthy cattle before and after induction with enrofloxacin. ProSpecT ELISA was used to quantify the Stx2 production by stx2-positive E. coli O157:H7/H7- isolates in native conditions (basal level) or after induction with enrofloxacin. Whereas only 15.2% of the E. coli O157:H7/H7- strains studied displayed significant amounts of detectable Stx2 without induction, most of them were shown to be inducible, and at various levels, in presence of subinhibitory concentrations of enrofloxacin. We demonstrated the capability of a highly elevated proportion of stx2-positive, but constitutively Stx2-negative, E. coli O157:H7/H7- isolates from healthy cattle to produce significant levels of Shiga toxin Stx2 in presence of subtherapeutic concentrations of enrofloxacin, an antibiotic of the fluoroquinolones family only licensed for veterinary use. This study documents the risk that bovine-associated Shiga toxin producing E. coli isolates may become more frequently pathogenic to humans as a side-effect of the increasing use of veterinary fluoroquinolones in the oral treatment of food animals like cattle or ...Continue Reading

References

Nov 1, 1995·Annals of Internal Medicine·C Su, L J Brandt
Oct 1, 1994·Epidemiology and Infection·D D HancockM G Paros
Mar 1, 1997·The Journal of Infectious Diseases·T E BesserP I Tarr
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Jan 29, 1999·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K GrifF Allerberger
Mar 27, 1999·Journal of Bacteriology·A MatsushiroT Honda
Feb 11, 2000·The Journal of Infectious Diseases·X ZhangD W Acheson
Jan 3, 2001·Applied and Environmental Microbiology·H KobayashiK Yamamoto
Jan 11, 2005·Applied and Environmental Microbiology·Wenlan ZhangHelge Karch
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Sylvia HeroldHerbert Schmidt
Feb 24, 2006·Microbiology and Immunology·Tsutomu KoitabashiMitsuaki Nishibuchi
Jul 6, 2006·Applied and Environmental Microbiology·Nancy A CornickDavid W K Acheson

❮ Previous
Next ❯

Citations

Jan 1, 2013·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·W V Kern
Sep 10, 2013·Applied and Environmental Microbiology·Woo Kyung JungThomas E Besser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.