Characterization of infiltrating macrophages in high glucose-induced peritoneal fibrosis in rats

Molecular Medicine Reports
Wenxue HuXueqing Yu

Abstract

Alternatively activated macrophages (M2 macrophages) are involved in tissue remodeling and fibrosis, but their effects on peritoneal fibrosis (PF) induced by high glucose peritoneal dialysate have yet to be fully established. In this study, PF was induced in male Sprague-Dawley rats by intraperitoneal injection with Lactate-G4.25% dialysate (20 ml/rat/day) for 4 weeks. Control rats were given an intraperitoneal injection with saline. Establishment of the PF model was verified by Masson's trichrome and H&E staining. M1 macrophages (co-localization of CD68 and CCr7) and M2 macrophages (co-localization of CD68 and CD206) was assayed by immunofluorescence and immunohistochemistry. The levels of dialysate cytokines driving macrophage differentiation (including IFN-γ, IL-2 and IL-4) were detected by ELISA. The expression of transforming growth factor (TGF)-β, p-Smad3, p-Smad2/3 and Smad7 in M2 macrophages (co-localization of CD68, CD206 and TGF-β, or p-Smad3, or p-Smad2/3, or Smad7) was measured by immunofluorescence. We found that PF rats had significantly thicker peritoneal membranes compared to control rats, indicating the successful establishment of our PF model. Compared to controls, PF rats had more peritoneal macrophages (CD68...Continue Reading

Citations

Apr 22, 2014·Experimental and Molecular Pathology·Kavindra Kumara WijesunderaJyoji Yamate
Sep 30, 2020·Biomolecules·Vanessa MarchantMarta Ruiz-Ortega
May 24, 2018·Molecular Pharmaceutics·Michael B DeciJuliane Nguyen

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