Characterization of inhibitory T cells induced by an analog of type II collagen in an HLA-DR1 humanized mouse model of autoimmune arthritis.

Arthritis Research & Therapy
Masaru KimataAndrew H Kang

Abstract

We used DR1 transgenic mice and covalently linked DR1 multimers to characterize analog-specific inhibitory T cells in collagen-induced arthritis (CIA). Because of the low numbers of antigen-specific T cells in wild-type mice, functional T-cell studies in autoimmune arthritis have been challenging. The use of T-cell receptor (TCR) transgenic mice has provided useful information, but such T cells may not represent the heterogeneous T-cell response that occurs in natural settings. Our focus was to develop tools to identify and characterize the population of immunoregulatory T cells induced in wild-type mice by an analog peptide of CII259-273, which contains amino acid substitutions at positions 263 (N) and 266 (D) (analog peptide A12). DR1 multimers, developed by loading empty class II molecules with exogenous peptide, provide a method for visualizing antigen-specific T cells with flow cytometry. However, the low binding avidity of A12 for the major histocompatibility complex (MHC) made this strategy untenable. To overcome this problem, we generated DR1 multimers in which the analog peptide A12 was covalently linked, hoping that the low-avidity analog would occupy enough binding clefts to allow detection of the responsive T cells....Continue Reading

References

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Citations

May 20, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Linda K MyersAndrew H Kang
Oct 6, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Tomohiro ArikawaMitsuomi Hirashima
Nov 12, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeoung-Eun ParkLinda K Myers
Oct 4, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chike O AbanaSimon A Mallal
Aug 28, 2020·Drug Metabolism Reviews·Takeshi SusukidaKousei Ito
Apr 21, 2018·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jeoung-Eun ParkLinda K Myers

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Methods Mentioned

BETA
transgenic
ELISA
flow cytometry
enzyme-linked immunoassay

Software Mentioned

FlowJo

Related Concepts

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