Characterization of injury incurred by Escherichia coli upon freeze-drying.

Journal of Bacteriology
T J Sinskey, G J Silverman

Abstract

When cells of Escherichia coli ML30 were suspended in 2% gelatin and frozen at -40 C, no appreciable metabolic damage or death occurred. After freeze-drying for 8 hr at a platen temperature of 49 C and rehydration with a mineral salts medium, survival of the cells was 0.6%. Metabolic damage of the survivors was found to be 23%. Permeability alterations were detected by several criteria. Freeze-dried cells were susceptible to antibiotics normally ineffective against E. coli and leakage of ribonucleic acid (RNA) occurred. Analysis of ribosomal extracts of rehydrated freeze-dried cells demonstrated the presence of appreciable degradation products. Permeability alterations were shown to be reversible by the observation that antibiotic susceptibility was a time-dependent process and that the gratuitous inducer of beta-galactosidase was not concentrated by freeze-dried cells until the injured cells had been incubated in a nutrient medium for 300 min or more. At approximately the same time, metabolic damage was repaired. RNA synthesis preceded protein synthesis by about 150 min, and deoxyribonucleic acid synthesis occurred with the resumption of normal growth. This was interpreted to be the result of repair of RNA taking place before ...Continue Reading

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Citations

Nov 1, 1993·The Journal of Applied Bacteriology·J J TheunissenM F Michel
Feb 5, 2013·Applied Microbiology and Biotechnology·Song WenfengNeil Pasco
Feb 1, 1980·The Journal of Applied Bacteriology·M D Breese, R J Sharp
Dec 8, 2005·Applied and Environmental Microbiology·Chung K MarstonTanja Popovic
Feb 1, 1979·Applied and Environmental Microbiology·K S Przybylski, L D Witter
Mar 1, 1979·Applied and Environmental Microbiology·Y TanakaT Miwatani
Nov 1, 1976·Applied and Environmental Microbiology·W W Lanz, P A Hartman
Aug 1, 1970·Applied Microbiology·D A Mossel, M A Ratto
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