Characterization of interleukin-1 receptor antagonist isoform expression in the brain of lipopolysaccharide-treated rats

Neuroscience
Karine PalinJ Lestage

Abstract

The endogenous interleukin-1 receptor antagonist is the natural inhibitor of the biological effects of interleukin-1 during inflammation. Interleukin-1 receptor antagonist refers to three isoforms: one secreted and two intracellular forms (types I and II). The objective of the present study was to investigate the expression of interleukin-1 receptor antagonist isoforms in the rat brain in vivo in response to an i.p. injection of lipopolysaccharide. The interleukin-1 receptor antagonist was studied at the messenger and protein levels by reverse transcription-polymerase chain reaction and western blot analysis, respectively. Interleukin-1 receptor antagonist messenger RNA was constitutively expressed in the brain and its expression increased in response to lipopolysaccharide. The three interleukin-1 receptor antagonist protein isoforms were up-regulated after lipopolysaccharide treatment in a time-dependent manner. Their relative expression differed according to the isoform and brain region studied. Double immunofluorescence staining revealed interleukin-1 receptor antagonist positive neurons and microglia in hippocampus 24h after lipopolysaccharide stimulation. These results demonstrate for the first time that brain cells are ab...Continue Reading

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Citations

Jan 6, 2006·Epilepsia·James G Heida, Quentin J Pittman
Jan 16, 2008·Physiological Reviews·Tihomir Paul Obrenovitch
Nov 26, 2008·Journal of Leukocyte Biology·Bart R TambuyzerEtienne J Nouwen

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