Characterization of Intestinal Lactobacillus reuteri Strains as Potential Probiotics

Probiotics and Antimicrobial Proteins
Tejinder Pal SinghSuman Kapila

Abstract

This study was conducted to evaluate the probiotic properties of Lactobacillus reuteri isolated from human infant feces (less than 3 months). Out of thirty-two representative L. reuteri strains isolated from the infant human feces, nine isolates (i.e. LR5, LR6, LR9, LR11, LR19, LR20, LR25, LR26 and LR34) showed survival in acid, bile and simulated stomach-duodenum passage conditions, indicating their high tolerance to gastric juice, duodenal juice and bile environments. The nine isolates did not show strong hydrophobic properties because the percentages of adhesion to the apolar solvent, n-hexadecane, did not exceed 40%, showing that their surfaces were rather hydrophilic. Functionality of these nine probiotic isolates was supported by their antagonistic activity and their ability to deconjugate bile salts. The safety of the nine indigenous L. reuteri isolates was supported by the absence of transferable antibiotic resistance determinants, DNase activity, gelatinase activity and hemolysis. The results obtained so far suggest that the nine strains are resistant to low pH, bile salts and duodenum juice, so they could survive when passing through the upper part of the gastrointestinal tract and fulfill their potential probiotic ac...Continue Reading

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Citations

Nov 3, 2012·World Journal of Microbiology & Biotechnology·Zhihui YuZhennai Yang
Sep 30, 2014·International Journal of Food Sciences and Nutrition·Tejinder Pal SinghGurpreet Kaur
May 31, 2018·Probiotics and Antimicrobial Proteins·Jyoti ChoudharyShrivardhan Dheeman
Nov 8, 2016·Frontiers in Microbiology·Ruby YadavPratyoosh Shukla
Apr 6, 2017·Frontiers in Microbiology·Tejinder P SinghRavinder K Malik
Jun 20, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Xuefang GuanBin Lin
Aug 25, 2017·Probiotics and Antimicrobial Proteins·Thatyane Mariano Rodrigues de AlbuquerqueEvandro Leite de Souza
Jul 19, 2019·Frontiers in Microbiology·Rakesh SomashekaraiahM Y Sreenivasa
May 18, 2020·Probiotics and Antimicrobial Proteins·Hugo Calixto FonsecaRosane Freitas Schwan
Mar 3, 2020·Journal of Food Science and Technology·Youfei ShiBaoquan Li
Jan 5, 2021·World Journal of Microbiology & Biotechnology·Pooja Pradhan, Jyoti Prakash Tamang
Oct 14, 2020·Journal of Microbiology and Biotechnology·Jeong Eun KangHo Jin Heo
May 11, 2021·Journal of Microbiological Methods·Jesús A Salas-TovarDavid R Sepulveda
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Matteo Dell'AnnoLuciana Rossi

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