PMID: 8604302Feb 15, 1996Paper

Characterization of intronic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plumbaginifolia

Nucleic Acids Research
M GniadkowskiW Filipowicz

Abstract

Introns of nuclear pre-mRNAs in dicotyledonous plants, unlike introns in vertebrates or yeast, are distinctly rich in A+U nucleotides and this feature is essential for their processing. In order to define more precisely sequence elements important for intron recognition in plants, we investigated the effects of short insertions, either U-rich or A-rich, on splicing of synthetic introns in transfected protoplast of Nicotiana plumbaginifolia. It was found that insertions of U-rich (sequence UUUUUAU) but not A-rich (AUAAAAA) segments can activate splicing of a GC-rich synthetic infron, and that U-rich segments, or multimers thereof, can function irrespective of the site of insertion within the intron. Insertions of multiple U-rich segments, either at the same or different locations, generally had an additive, stimulatory effect on splicing. Mutational analysis showed that replacement of one or two U residues in the UUUUUAU sequence with A or C residues had only a small effect on splicing, but replacement with G residues was strongly inhibitory. Proteins that interact with fragments of natural and synthetic pre-mRNAs in vitro were identified in nuclear extracts of N.plumbaginifolia by UV cross- linking. The profile of cross-linked ...Continue Reading

References

Jan 1, 1992·Progress in Nucleic Acid Research and Molecular Biology·R M Sinibaldi, I J Mettler
Mar 1, 1991·Plant Molecular Biology·A G HuntN H Chua
Jan 1, 1990·Methods in Enzymology·G J GoodallW Filipowicz
Jan 1, 1991·Annual Review of Cell Biology·M R Green
Nov 1, 1988·Trends in Genetics : TIG·T Blumenthal, J Thomas
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J L ManleyM L Gefter
Jan 1, 1994·Annual Review of Genetics·H D Madhani, C Guthrie
Apr 1, 1995·Genes & Development·J G Umen, C Guthrie
Jan 1, 1993·Plant Molecular Biology·C G Simpson, J W Brown
Dec 1, 1994·Plant Molecular Biology·J C Carle-UriosteV Walbot
May 1, 1994·Genes & Development·K R Luehrsen, V Walbot
Jan 1, 1994·Progress in Nucleic Acid Research and Molecular Biology·K R LuehrsenV Walbot
Aug 1, 1993·Molecular and Cellular Biology·H LouM A Schuler
Jan 1, 1993·Annual Review of Biochemistry·G DreyfussC G Burd
Mar 1, 1993·Molecular and Cellular Biology·A J McCulloughM A Schuler
Feb 25, 1993·Nucleic Acids Research·R ConradT Blumenthal

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Citations

Oct 1, 1996·Plant Molecular Biology·M Sugita, M Sugiura
Mar 31, 2000·Trends in Plant Science·Z J LorkovićW Filipowicz
Apr 1, 2000·RNA·D LibriM Rosbash
Dec 1, 1996·Nature Genetics·H J WuR Rouzé
Sep 23, 1998·The Plant Journal : for Cell and Molecular Biology·C G SimpsonJ W Brown
Sep 23, 1998·The Plant Journal : for Cell and Molecular Biology·M IsshikiK Shimamoto
Sep 6, 2000·The Plant Journal : for Cell and Molecular Biology·M IsshikiK Shimamoto
Sep 22, 2010·Journal of Experimental Botany·Laura MorelloDiego Breviario
Jun 17, 2011·Journal of Experimental Botany·Eduardo F MufarregeGraciela C Curi
Sep 25, 2004·Nucleic Acids Research·Marie TouchonClaude Thermes
Sep 2, 1998·The Plant Cell·R W Sablowski, E M Meyerowitz
May 25, 2002·Molecular and Cellular Biology·Mark H L LambermonZdravko J Lorković
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·J. W. S. Brown, C. G. Simpson
Dec 11, 2008·PLoS Biology·Francesco Catania, Michael Lynch
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Craig G SimpsonJohn W S Brown
Mar 26, 1998·Journal of Molecular Biology·V BrendelV Walbot
Apr 7, 2017·The Journal of Biological Chemistry·Witold Filipowicz
Jul 20, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·E BolloL Rossi
Dec 14, 1999·The Journal of Biological Chemistry·M Golovkin, A S Reddy
Aug 28, 2016·Genome Biology and Evolution·Amir SzitenbergDavid H Lunt
May 28, 2019·Frontiers in Plant Science·Hyo-Young ParkJeong-Kook Kim
Apr 1, 1997·Molecular and Cellular Biology·Z J LorkovićR Oelmüller

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