Characterization of ionotropic glutamate receptors in insect neuro-muscular junction

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
I M FedorovaD B Tikhonov

Abstract

Pharmacological properties of ionotropic glutamate receptors from Calliphora vicina larvae neuro-muscular junction (C. vicina iGlurs) were studied by two-electrode voltage-clamp technique. Characteristics of the ion channel pore were analyzed using a 26-member series of channel blockers, which includes mono- and dicationic derivatives of adamantane and phenylcyclohexyl. Structure-activity relationships were found to be markedly similar to the Ca2+-permeable AMPA receptors (AMPAR) but not NMDA receptors (NMDAR) channel subtype seen in vertebrates. Like AMPARs the channels of C. vicina iGlurs are sensitive mainly to dicationic compounds with 6-7 spacers between hydrophobic headgroup and terminal aminogroup. Study of the voltage dependence of block demonstrated that, like AMPARs, the C. vicina iGlur channels, are permeable to organic cations with dimensions exceeding 10 A. Concentration dependence of block suggests the presence of two distinct channel populations with approximately 20-fold different sensitivity to cationic blockers. The recognition domain properties are more complex. Besides glutamate, the channels can be activated by kainate, quisqualate and domoate. Competitive antagonists of AMPAR and NMDAR are virtually inacti...Continue Reading

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S Isaacson, R A Nicoll
Jan 11, 1990·Toxicon : Official Journal of the International Society on Toxinology·M BruceP N Usherwood
May 17, 1994·European Journal of Pharmacology·F A TavernaJ F MacDonald
Jan 1, 1995·Invertebrate Neuroscience : in·H L SudanP N Usherwood
Feb 7, 1998·Journal of Neurophysiology·M J BrierleyP R Benjamin
May 23, 1998·The Journal of Physiology·R Bähring, M L Mayer
Nov 23, 2000·Journal of Medicinal Chemistry·K StrømgaardJ W Jaroszewski
Apr 11, 2002·Neuroscience and Behavioral Physiology·L G MagazanikE V Frolova
Sep 13, 2003·Journal of Neurochemistry·K V BolshakovL G Magazanik
Apr 7, 2004·Toxicon : Official Journal of the International Society on Toxinology·Ian R Mellor, Peter N R Usherwood
Jul 6, 2005·Neuropharmacology·K V BolshakovL G Magazanik
Dec 2, 2006·International Review of Neurobiology·Aaron DiAntonio
Apr 25, 2007·Molecular Membrane Biology·Denis B Tikhonov

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Citations

May 23, 2012·The Journal of Biological Chemistry·Lucia Kuhn-NentwigLev G Magazanik
Jan 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jing ZhangDon B Gammon
Jan 17, 2021·International Journal of Molecular Sciences·Lindsay M AchzetDarrell A Jackson
Dec 12, 2020·Scientific Reports·Agnieszka KlupczynskaJan Matysiak

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