PMID: 8952954Dec 1, 1996Paper

Characterization of K+ channels in the basolateral membrane of rat tracheal epithelia

The Journal of Membrane Biology
T H HwangJ Jung

Abstract

To study K+ channels in the basolateral membrane of chloride-secreting epithelia, rat tracheal epithelial monolayers were cultured on permeable filters and mounted into an Ussing chamber system. The mucosal membrane was permeabilized with nystatin (180 microg/ml) in the symmetrical high K+ (145 mm) Ringer solution. During measurement of the macroscopic K+ conductance properties of the basolateral membrane under a transepithelial voltage clamp, we detected at least two types of K+ currents: one is an inwardly rectifying K+ current and the other is a slowly activating outwardly rectifying K+ current. The inwardly rectifying K+ current is inhibited by Ba2+. The slowly activating K+ current was potentiated by cAMP and inhibited by clofilium, phorbol 12-myristate 13-acetate (PMA) and lowering temperature. This is consistent with the biophysical characteristics of ISK channel. RT-PCR analysis revealed the presence of ISK cDNA in the rat trachea epithelia. Although 0.1 mM Ba2+ only had minimal affect on short-circuit current (Isc) induced by cAMP in intact epithelia, 0.1 mM clofilium strongly inhibited it. These results indicate that ISK might be important for maintaining cAMP-induced chloride secretion in the rat trachea epithelia.

Citations

Jul 1, 1998·Pharmacology & Toxicology·J S JungS H Lee
Nov 2, 2001·Biochemical and Biophysical Research Communications·J S JeongT H Hwang
Feb 1, 2002·Biochemical and Biophysical Research Communications·Giuliano MeyerMarkus Paulmichl
Feb 5, 2002·The Journal of Physiology·Elizabeth A Cowley, Paul Linsdell
Nov 6, 2004·Neuropharmacology·Zoe A McCrossan, Geoffrey W Abbott
Aug 2, 2005·The Journal of Pharmacology and Experimental Therapeutics·Huiling LiuJerry M Farley
Feb 8, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Richard Warth, Jacques Barhanin
Feb 24, 2001·American Journal of Physiology. Cell Physiology·S DemolombeD Escande

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