Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Fangjun ChengWenjie Shan

Abstract

Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93.4% of the strains. Of which, TEM was the most prevalent (93.4%), followed by CTX-M and SHV were 57.6% and 39.4%, respectively. A main mutation pattern of quinoloneresistance-determining region, Thr83-Ieu and Asp87-Asn in gyrA and Ser87-Ile in parC, was detected in 33 K. pneumoniae isolates. All the isolates harbored at least two virulence factor genes, with ureA (97.0%) and wabG (91.0%) exhibiting high carriage rates in 33 K. pneumoniae isolates. MLST revealed 7 sequence types, of which 3 STs (2541, 2581 and 2844) were newly assigned. Using eBURST, ST2844 and ST2541 were assigned to new clonal complex 2844. Our study provides evidence and biological characteristics of K. pneumoniae isolates from cattle upper res...Continue Reading

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Citations

May 17, 2019·Clinical Microbiology Reviews·Thomas A Russo, Candace M Marr
Mar 12, 2020·Veterinary World·Ekaterina LenchenkoAlfia Ibragimova
Feb 14, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Jesús Silva-SanchezUlises Garza-Ramos
Dec 29, 2020·Journal of Dairy Science·Wenpeng ZhaoJian Gao
Jun 3, 2021·International Journal of Molecular Sciences·Olivia M GrünzweilIgor Loncaric
May 25, 2021·The Journal of Veterinary Medical Science·Tetsuya KomatsuTomoyuki Shibahara
Jun 5, 2020·Ecotoxicology and Environmental Safety·João Pedro Rueda FurlanEliana Guedes Stehling

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Datasets Mentioned

BETA
MEGA7.0
CP018306
MG758055-MG758108
MG869692-MG869703

Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

Clustal
MAFFT
eBURST
- LINSI
MEGA7
BLAST ( Basic Local Alignment Search Tool )
BLAST
MAFFT Multiple Sequence Alignment
MEGA

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