Characterization of KPC-Producing Serratia marcescens in an Intensive Care Unit of a Brazilian Tertiary Hospital

Frontiers in Microbiology
Roumayne L FerreiraMaria-Cristina da Silva Pranchevicius

Abstract

Serratia marcescens has emerged as an important opportunistic pathogen responsible for nosocomial and severe infections. Here, we determined phenotypic and molecular characteristics of 54 S. marcescens isolates obtained from patient samples from intensive-care-unit (ICU) and neonatal intensive-care-unit (NIUC) of a Brazilian tertiary hospital. All isolates were resistant to beta-lactam group antibiotics, and 92.6% (50/54) were not susceptible to tigecycline. Furthermore, 96.3% showed intrinsic resistance to polymyxin E (colistin), a last-resort antibiotic for the treatment of infections caused by MDR (multidrug-resistant) Gram-negative bacteria. In contrast, high susceptibility to other antibiotics such as fluoroquinolones (81.5%), and to aminoglycosides (as gentamicin 81.5%, and amikacin 85.2%) was found. Of all isolates, 24.1% were classified as MDR. The presence of resistance and virulence genes were examined by PCR and sequencing. All isolates carried KPC-carbapenemase (bla KPC ) and extended spectrum beta-lactamase bla TEM genes, 14.8% carried bla OXA- 1, and 16.7% carried bla CTX-M- 1 group genes, suggesting that bacterial resistance to β-lactam antibiotics found may be associated with these genes. The genes SdeB/HasF ...Continue Reading

References

May 1, 1991·The Journal of Hospital Infection·P U KrishnanR Macaden
May 1, 1980·Journal of Medical Microbiology·V M YoungM J Morris
Nov 22, 1997·Journal of Medical Microbiology·A Hejazi, F R Falkiner
Dec 23, 1999·Current Opinion in Microbiology·G M Fraser, C Hughes
Apr 27, 2000·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·J Raymond, Y Aujard
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·G S Chilcott, K T Hughes
Mar 19, 2002·Emerging Infectious Diseases·Wen-Chien KoVictor L Yu
Jun 29, 2002·Diagnostic Microbiology and Infectious Disease·Wang-Huei ShengWei-Chuan Hsieh
May 6, 2003·Diagnostic Microbiology and Infectious Disease·Wen-Liang YuRonald N Jones
Jul 3, 2003·The Journal of Antimicrobial Chemotherapy·Jing ChenTomofusa Tsuchiya
Mar 24, 2004·Archives of Medical Research·José Antonio Enciso-MorenoRafael Coria-Jiménez
Oct 16, 2004·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Vladana MilisavljevicLisa Saiman
Dec 4, 2004·Nature Medicine·Stuart B Levy, Bonnie Marshall
Jan 7, 2005·Journal of Clinical Microbiology·Mimi HealyJames R Lupski
Mar 29, 2005·Antimicrobial Agents and Chemotherapy·Ayush Kumar, Elizabeth A Worobec
Aug 27, 2005·Canadian Journal of Microbiology·Ayush Kumar, Elizabeth A Worobec
Mar 7, 2006·The Journal of Hospital Infection·M D DavidA P Fraise
Apr 17, 2007·American Journal of Infection Control·Petra GastmeierHenning Rüden
Jun 22, 2007·Journal of Clinical Microbiology·K F AndersonJ B Patel
Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, Karen Bush
Jan 30, 2008·Antimicrobial Agents and Chemotherapy·Thierry NaasPatrice Nordmann
Apr 19, 2008·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Lisa L MaragakisTrish M Perl
May 2, 2008·Canadian Journal of Microbiology·Sanela Begic, Elizabeth A Worobec
Oct 14, 2008·Expert Review of Anti-infective Therapy·Matthew E FalagasArgyris Michalopoulos
Jan 15, 2010·The Journal of Antimicrobial Chemotherapy·Caroline DallenneGuillaume Arlet
Jan 21, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V MiriagouR Cantón
Aug 18, 2010·Infection and Immunity·Chuan-Sheng LinHsin-Chih Lai
Oct 12, 2010·Journal of Global Infectious Diseases·Deepti Rawat, Deepthi Nair
Dec 24, 2010·Annals of the New York Academy of Sciences·Karen Bush
Jul 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A-P MagiorakosD L Monnet
Aug 24, 2011·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Oct 7, 2011·Clinical Microbiology Reviews·Steven D Mahlen
Oct 18, 2011·Emerging Infectious Diseases·Patrice NordmannLaurent Poirel

❮ Previous
Next ❯

Citations

Jul 1, 2021·Journal of Biomolecular Structure & Dynamics·Ligia Carolina da Silva PradoSiomar de Castro Soares

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
genotyping

Software Mentioned

BioNumerics
Blastn
Bioedit

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.