PMID: 3083865Apr 25, 1986Paper

Characterization of L-threonine and L-glutamine transport in murine P388 leukemia cells in vitro. Presence of an N-like amino acid transport system

Biochimica Et Biophysica Acta
P Lazarus, L C Panasci

Abstract

The transport of L-threonine and L-glutamine into murine P388 leukemia cells has been characterized. Threonine appears to be a specific substrate for a Na+-dependent amino acid transport system similar to system ASC of the HTC hepatoma cell. Threonine transport is uninhibited by 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid and alpha-(methylamino)isobutyric acid, shows a pattern of transport similar to that seen in HTC hepatoma cells over the pH range of 5.5-7.5, and is inhibited by L-serine and L-cysteine. Approximately two-thirds of glutamine transport into P388 cells also appears to enter P388 cells via this ASC-analogous system. However, based upon (a) inhibition studies with threonine (where the K1 of threonine inhibition of glutamine transport was 7-fold the Km of threonine transport), (b) inhibition analysis of glutamine transport with various amino acids and amino acid analogues, and (c) different patterns of transport between threonine and glutamine over the pH range of 5.5-7.5, approximately one-third of glutamine transport can be attributed to a second Na+-dependent amino acid transport system. This system appears to be similar to the system N of rat hepatocytes. Glutamine and threonine do not appear to enter P388 c...Continue Reading

References

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Citations

Oct 18, 2000·Pathology Oncology Research : POR·P Bhattacharya, P Maity
Sep 1, 1988·The Journal of Clinical Investigation·D B Learn, E L Thomas
Dec 9, 1991·Biochimica Et Biophysica Acta·O BussolatiG C Gazzola
May 2, 1998·Brain Research·J XiangR F Keep
Jan 12, 1999·The Journal of Physiology·G A BarkerJ C Ellory
Apr 11, 2014·PloS One·José Antonio FizJuan Ruiz
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Eiichi SakuraiYorihisa Tanaka

Related Concepts

2-aminoisobutyric acid,14C-labeled
Aminoisobutyric Acids
Metazoa
D-Glutamine
Hydrogen-Ion Concentration
Leukemia P388
Leukemia, Experimental
Mice, Inbred BALB C
Mice, Inbred DBA
Sodium

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