Characterization of Lactobacillus sake isolates from dry-cured sausages by restriction fragment length polymorphism analysis of the 16S rRNA gene

Journal of Applied Microbiology
Y SanzJ Hernández

Abstract

Lactobacillus sake strains originally isolated from dry-fermented sausages were characterized by phenotypic and genotypic methods, including DNA-DNA hybridization, restriction fragment length polymorphism (RFLP), and 16S rDNA sequencing analysis, in order to establish their taxonomic position and relation to well defined reference species. Initially, isolates of Lact. sake showing a characteristic phenotype (melibiose-positive, maltose- and arabinose-negative) were identified by DNA-DNA hybridization. Subsequently, RFLP studies using EcoRI and HindIII as restriction enzymes, and cDNA from Escherichia coli or 16S rDNA from Lact. sake strains as probes, showed distinct polymorphism levels. Thus, EcoRI-digested DNA probed with cDNA from E. coli disclosed the presence of a unique cluster for the meat isolates tested, allowing their differentiation from the reference type strain. When HindIII-digested DNA was hybridized with the cDNA probe, strain-specific patterns were obtained, showing a higher discrimination power. Considerable strain differentiation was also observed when EcoRI and HindIII digests were hybridized with 16S rDNA probes. Finally, sequence analysis of the 16S rDNA from one isolate also revealed a certain degree of g...Continue Reading

Citations

Aug 9, 2005·International Journal of Food Microbiology·Cecilia FontanaGraciela Vignolo
Jan 14, 2000·International Journal of Food Microbiology·Y SanzF Toldrá
Mar 29, 2000·Journal of Applied Microbiology·R SatokariM L Suihko
Apr 3, 2001·Applied and Environmental Microbiology·Y Sanz, F Toldrá
Mar 28, 2002·Applied and Environmental Microbiology·Yolanda Sanz, Fidel Toldrá
Oct 19, 2006·International Journal of Food Microbiology·Kalliopi RantsiouLuca Cocolin
Feb 17, 2006·International Journal of Food Microbiology·Kalliopi Rantsiou, Luca Cocolin
Jan 30, 1999·Applied and Environmental Microbiology·S FaddaF Toldrá

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