PMID: 9421966Jan 9, 1998Paper

Characterization of lamprey rhodopsin by isolation from lamprey retina and expression in mammalian cells

Photochemistry and Photobiology
O HisatomiF Tokunaga

Abstract

A visual pigment was extracted from lamprey retina and was expressed in cultured mammalian cells (293S) using a cDNA fragment isolated from lamprey retina. The extracted pigment, a putative lamprey rhodopsin, had an absorption maximum at 503 nm. The recombinant lamprey rhodopsin, reconstituted with 11-cis-retinal, showed an absorption maximum at about 500 nm. Both pigments reacted with an anti-bovine rhodopsin antibody (Rh29), which recognizes the short photoreceptor cells in lamprey retina. Unlike rhodopsins of higher vertebrates, the lamprey rhodopsin bleached gradually in the presence of 100 mM hydroxylamine even in the dark. Our results suggest that, despite its high similarities with other vertebrate rhodopsins, lamprey rhodopsin has a character different from those of higher vertebrates.

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Citations

Nov 9, 2000·Progress in Retinal and Eye Research·T Ebrey, Y Koutalos
Sep 2, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Shaun P CollinDavid M Hunt
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mitsumasa KoyanagiAkihisa Terakita
Jun 11, 2015·Scientific Reports·Masataka YanagawaYoshinori Shichida
Jun 20, 2007·Photochemistry and Photobiology·David M HuntJames K Bowmaker
Apr 28, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wayne L DaviesShaun P Collin
Oct 3, 1998·The Journal of Biological Chemistry·M MaxB E Knox

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