PMID: 7543468Aug 1, 1995Paper

Characterization of late gene promoters of Chlamydia trachomatis

Journal of Bacteriology
M J FahrT P Hatch

Abstract

Chlamydiae possess an intracellular developmental cycle defined by the orderly interconversion of infectious, metabolically inactive elementary bodies and noninfectious, dividing reticulate bodies. Only a few stage-specific genes have been cloned and sequenced, including the late-stage cysteine-rich protein operon and two late-stage genes encoding histone-like proteins. The aims of this study were to identify additional late-stage genes of Chlamydia trachomatis, analyze the upstream DNA sequence of late genes, and determine the sigma factor requirement of late genes. Stage-specific RNA, made by chlamydiae isolated from host cells, was used to probe C. trachomatis genomic libraries. Two new late genes, designated ltuA and ltuB, were identified, cloned, and sequenced. The predicted peptides encoded by ltuA and ltuB do not bear strong homology to known proteins, and the function of the new late genes is not known. The 5' ends of the transcripts of ltuA, ltuB, the cysteine-rich protein operon, and the two histone-like genes (hctA and hctB) were mapped, and a consensus -10 promoter region of TATAAT was derived from their upstream DNA sequences. In vitro transcription from templates encoding the promoter regions of ltuA, ltuB, and hc...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·E Southern
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E PeraraJ N Engel
Jun 1, 1992·Journal of Bacteriology·M LonettoC A Gross
Oct 1, 1990·Infection and Immunity·R W CrenshawT P Hatch
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T HackstadtY Ying
Mar 1, 1991·Microbiological Reviews·J W Moulder
Dec 1, 1990·Journal of Bacteriology·J N EngelD Ganem
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W BaehrH D Caldwell
Jun 1, 1989·Journal of Bacteriology·S F Stoddard, M M Howe
Dec 1, 1988·Infection and Immunity·M R Plaunt, T P Hatch
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Mar 1, 1995·Microbiological Reviews·W G Haldenwang
Dec 1, 1994·Microbiological Reviews·W L BeattyG I Byrne
May 1, 1994·Trends in Microbiology·G McClarty
Jul 1, 1994·Canadian Journal of Microbiology·J E TamP B Wyrick
May 1, 1993·Journal of General Microbiology·M ComanducciG Ratti
Mar 1, 1993·Molecular Microbiology·S A MathewsT P Hatch
May 1, 1993·Journal of Bacteriology·D G Wichlan, T P Hatch
Mar 1, 1965·Journal of Bacteriology·J W MOULDERR R BRUBAKER

❮ Previous
Next ❯

Citations

Sep 14, 2000·The Journal of Biological Chemistry·C RoshickG McClarty
Nov 18, 2003·Molecular Microbiology·Hilda Hiu Yin Yu, Ming Tan
Nov 6, 2010·Nucleic Acids Research·Yasser M AbdelrahmanRobert J Belland
Feb 27, 2015·Frontiers in Cellular and Infection Microbiology·Emilie GauliardDaniel Ladant
Jan 5, 2006·Molecular Microbiology·Nicole A GrieshaberTed Hackstadt
May 6, 2003·Journal of Bacteriology·Tracy L NicholsonRichard S Stephens
Aug 26, 2000·Journal of Bacteriology·C S Schaumburg, M Tan
Oct 24, 2006·Journal of Bacteriology·P Scott Hefty, Richard S Stephens
Aug 29, 2006·Journal of Bacteriology·Li ShenYou-Xun Zhang
Jan 30, 2007·Microbiology·Masayuki MurataMutsunori Shirai
Jul 29, 2008·Journal of Bacteriology·Eike NiehusMing Tan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.