PMID: 9188583Jul 1, 1997Paper

Characterization of late gene transcripts expressed during vegetative replication of human papillomavirus type 31b

Journal of Virology
Michelle A Ozbun, C Meyers

Abstract

Human papillomaviruses (HPVs) are etiologic agents of anogenital cancers. The lack of an efficient in vitro system with which to study the differentiation-dependent viral life cycle has impeded most investigations of viral transcription and gene expression. The CIN-612 clone 9E cell line latently maintains episomal copies of HPV type 31b (HPV31b). The complete replicative life cycle of HPV31b can be studied by using the organotypic (raft) culture system. A number of spliced HPV31b early gene transcripts and two late gene transcripts have been described in studies using the raft system. An HPV31b early promoter, P97, and a differentiation-induced promoter, P742, have been characterized by using this system. In this study, we used the raft system to analyze the temporal expression patterns of HPV31b late gene transcripts during the viral life cycle. The expression of late RNAs peaked at day 12 after lifting to the air-liquid interface; the levels then declined dramatically by day 16. The peak of late RNA expression was coincident with the appearance of virus particles in the raft tissues. We characterized transcripts with the potential to encode late gene products, including 19 RNAs containing the L1 region and 4 RNAs containing ...Continue Reading

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Citations

Jan 19, 1999·Annual Review of Microbiology·H Kasamatsu, A Nakanishi
Nov 24, 1999·The Journal of General Virology·T H BraunsteinB Norrild
Sep 5, 2002·Journal of Virology·Jennifer L Bromberg-White, Craig Meyers
May 17, 2014·Pathology Oncology Research : POR·Mohammed Amjed AlsaeghSheng Rong Zhu
Aug 29, 2016·BMC Biotechnology·Hyoung Jin KimHong-Jin Kim
Jun 24, 2020·Pathogens·Michelle Mac, Cary A Moody
Nov 16, 2005·Reviews in Medical Virology·Christy M Hebner, Laimonis A Laimins
Apr 23, 2002·Journal of Virology·Craig MeyersKathrin U Jansen
Feb 26, 2005·Journal of Virology·Jason M Bodily, Craig Meyers
Mar 12, 2013·Nature Reviews. Microbiology·Cecilia Johansson, Stefan Schwartz
Jan 11, 2020·Seminars in Immunopathology·Megan BurleyJoanna L Parish
Nov 5, 2002·Journal of Virology·Kong-Nan Zhao, Ian H Frazer
Jan 31, 2015·Evolution, Medicine, and Public Health·Ignacio G Bravo, Marta Félez-Sánchez
Sep 10, 2014·Expert Review of Anti-infective Therapy·Eric J Ryndock, Craig Meyers
Sep 20, 2002·Journal of Virology·Woei Ling PehJohn Doorbar
Jul 24, 2012·Biochemical Society Transactions·Tetyana Klymenko, Sheila V Graham
May 2, 2009·Journal of Dental Research·M J Conway, C Meyers
Aug 8, 2015·Viruses·Jennifer Biryukov, Craig Meyers

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