PMID: 7546247Apr 1, 1995Paper

Characterization of latent transforming growth factor-beta from human seminal plasma

American Journal of Reproductive Immunology : AJRI
M Nocera, T M Chu

Abstract

Human seminal plasma is known to exhibit immunosuppressive activity. Transforming growth factor beta (TGF-beta) has been identified as an immunosuppressive factor in human seminal plasma. Biologically active TGF-beta represents a family of 25-kDa homodimeric proteins linked with disulfide bonds. TGF-beta associates with high molecular weight proteins noncovalently to form a type of latency that is biologically inactive. Quantitative distribution of active form of TGF-beta versus inactive latent form of of TGF-beta, and mechanism of the TGF-beta activation in human seminal plasma remain to be elucidated. To characterize seminal plasma latent form of TGF-beta, including its concentration, and the mechanism underlying the activation of TGF-beta. Gel filtrations on ACA-34 and Biogel P-60 were used to fractionate seminal plasma. TGF-beta was measured by enzyme immunoassay using antibodies specific for TGF-beta 1 and TGF-beta 2, respectively. Radioreceptor assay with recombinant human [125I]-TGF-beta 1 was applied to qualitatively identify TGF-beta 1. Kinetic experiments with various pH, temperature and time, along with protease inhibitors, were performed to delineate the activation mechanism of latent TGF-beta 1. Human seminal plasm...Continue Reading

References

Feb 1, 1976·Fertility and Sterility·K S Moghissi
Mar 1, 1991·The Journal of Clinical Investigation·J KekowM Lotz
Jan 1, 1990·Annals of the New York Academy of Sciences·P ten DijkeC Pieler
Feb 1, 1987·Fertility and Sterility·N J Alexander, D J Anderson
Jan 1, 1987·Methods in Enzymology·L M Wakefield
May 1, 1987·Journal of Cellular Physiology·R A Fava, D B McClure
Jan 1, 1983·Hybridoma·L D PapsideroT M Chu
Jun 23, 1993·Annals of the New York Academy of Sciences·M Lotz, P Seth
Jul 1, 1993·Journal of Reproductive Immunology·P PöllänenO Söder
Aug 1, 1993·American Journal of Reproductive Immunology : AJRI·M Nocera, T M Chu
Dec 23, 1993·Biochimica Et Biophysica Acta·P R Segarini
Sep 29, 1962·Annals of the New York Academy of Sciences·W T POMMERENKE

❮ Previous
Next ❯

Citations

May 24, 2005·Cell and Tissue Research·Sarah A Robertson
Aug 1, 1997·American Journal of Reproductive Immunology : AJRI·L VeselskýB Zelezná
Jul 14, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S MaedaB D Boyan
Aug 15, 2009·Journal of Reproductive Immunology·Ee Min KhoUNKNOWN SCOPE Consortium
Nov 10, 2013·Mediators of Inflammation·Alejandro EscobarMónica Imarai
Jul 15, 2015·Reproductive Biology and Endocrinology : RB&E·John C ChapmanSandra D Michael
Nov 18, 2016·American Journal of Respiratory Cell and Molecular Biology·Christie A OjiakuReynold A Panettieri
Jan 22, 2017·Cold Spring Harbor Perspectives in Biology·Shomyseh SanjabiMing O Li
Oct 31, 2012·Molecular Reproduction and Development·Brooke A Laflamme, Mariana F Wolfner
Mar 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Nadia GopichandranNicolas M Orsi
Oct 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Shubash Chandra DasYukinori Yoshimura
Jul 13, 2002·Journal of Medical Virology·Masako Moriuchi, Hiroyuki Moriuchi
Aug 6, 2003·FEMS Immunology and Medical Microbiology·Kenneth W Beagley, Christine M Gockel
Jun 8, 1999·Human Reproduction·B LorasM Benahmed
Sep 19, 2019·BioMed Research International·Justyna SzczykutowiczMirosława Ferens-Sieczkowska
Jun 19, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·David J SharkeySarah A Robertson
Aug 22, 2018·International Journal of Molecular Sciences·Shunjiro YamakawaNoriyasu Hosoya
Dec 21, 2018·Frontiers in Immunology·Cosnet L RametseJo-Ann S Passmore
Oct 19, 2002·Journal of Reproductive Immunology·Sarah A RobertsonKelton P Tremellen
Dec 15, 2019·Journal of Reproductive Immunology·Line Lynge NilssonThomas Vauvert F Hviid
Jul 17, 2007·Biochimica Et Biophysica Acta·Georgios Pampalakis, Georgia Sotiropoulou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.