PMID: 6970163Nov 1, 1980Paper

Characterization of lectin-induced cellular cytotoxicity mediated by mouse spleen cells and the role of lymphotoxin

Immunology
J Sawada, T Osawa

Abstract

The cellular cytotoxicity mediated by mouse spleen cells in the presence of mitogenic or non-mitogenic lectins was established under serum-free conditions and characterized. Compared with the lectin-induced cellular cytotoxicity (LICC) in the guinea-pig, the activity of lymphotoxin (LT) released into the murine LICC assay cultures was very low. However, a positive correlation was found between the strength of LICC and the LT activity released into the supernatants. Moreover, the addition of puromycin, a potent enhancing reagent of guinea-pig LT activity, markedly promoted the LICC when added 4 h after the initiation of the LICC culture. These data, taken together, suggest that LT acts as an effector molecule in the murine LICC systems as well as in the guinea-pig LICC systems. Properties of the effector cell populations mediating LICC were investigated by depletion of plastic-adherent or nylon-wool adherent cells, by treatment of spleen cells with anti-T-cell sera and complement, and by use of nude mouse spleen cells. The results obtained suggest that both concanavalin A and phytohaemagglutinin-P can induce nylon-wool non-adherent T-cell mediated LICC, phytohaemagglutinin-W was found to be capable of inducing both the nylon-woo...Continue Reading

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.