Characterization of Lef-1 promoter segments that facilitate inductive developmental expression in skin

The Journal of Investigative Dermatology
Xiaoming LiuJ F Engelhardt

Abstract

Lymphoid Enhancer Factor 1 (Lef-1) is an important developmental transcription factor required for the inductive formation of several epithelial-derived organs including hair follicles. Inductive expression of Lef-1 mRNA is tightly regulated during embryo development, suggesting the involvement of a highly regulated promoter. In vitro analysis of the Lef-1 gene has demonstrated the existence of at least two spatially distinct promoters with multiple transcriptional start sites that are responsive to the canonical Wnt/beta-catenin pathway. Regions of the Lef-1 promoter required for inductive regulation in vivo, however, have yet to be determined. To this end, we utilized LacZ-reporter transgenic mice to define segments of the human Lef-1 promoter capable of reproducing mesenchymal- or epithelial-restricted transcriptional patterns of Lef-1 expression during hair and vibrissa follicle development. These studies have revealed that a 110 bp Wnt/beta-catenin-responsive element, contained within a minimal 2.5 kb Lef-1 promoter, plays an important role in regulating mesenchymal, and potentially epithelial, expression during follicle development in mouse embryos. This 2.5 kb Lef-1 promoter also demonstrated inductive mesenchymal expres...Continue Reading

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Citations

Nov 27, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaoming LiuJohn F Engelhardt
Aug 24, 2010·The Journal of Biological Chemistry·Genta PlasariAlessandra Calabrese
Jul 1, 2006·Molecular and Cellular Biology·Tony W-H LiMarian L Waterman
Aug 31, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaoming LiuJohn F Engelhardt
Jul 13, 2004·The Journal of Investigative Dermatology·Randall B Widelitz
May 4, 2011·Annals of the New York Academy of Sciences·Diana K SarkoRoger L Reep
Nov 5, 2014·Histochemistry and Cell Biology·Alexandra RippaEkaterina Vorotelyak
Mar 6, 2007·Developmental Biology·Ryan R DriskellJohn F Engelhardt
Jul 3, 2010·Molecular Oncology·Kate D Sutherland, Anton Berns
Oct 4, 2017·Diagnostic Cytopathology·Alessandra C SchmittMomin T Siddiqui
Jan 9, 2019·The International Journal of Lower Extremity Wounds·Binghui LiJianghai Chen
Aug 5, 2010·Medical Molecular Morphology·Yasuyuki AmohRobert M Hoffman
Jun 15, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ryan R DriskellJohn F Engelhardt
Feb 4, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weiliang XieJohn F Engelhardt
Feb 25, 2005·Journal of Cell Science·Usha VadlamudiBrad A Amendt

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