PMID: 9529084Apr 7, 1998Paper

Characterization of leptospiral outer membrane lipoprotein LipL36: downregulation associated with late-log-phase growth and mammalian infection

Infection and Immunity
David A HaakeCarole A Bolin

Abstract

We report the cloning of the gene encoding a 36-kDa leptospiral outer membrane lipoprotein, designated LipL36. We obtained the N-terminal amino acid sequence of a staphylococcal V8 proteolytic-digest fragment in order to design an oligonucleotide probe. A Lambda-Zap II library containing EcoRI fragments of Leptospira kirschneri DNA was screened, and a 2.3-kb DNA fragment which contained the entire structural lipL36 gene was identified. Several lines of evidence indicate that LipL36 is lipid modified in a manner similar to that of LipL41, a leptospiral outer membrane lipoprotein we described in a previous study (E. S. Shang, T. A. Summers, and D. A. Haake, Infect. Immun. 64:2322-2330, 1996). The deduced amino acid sequence of LipL36 would constitute a 364-amino-acid polypeptide with a 20-amino-acid signal peptide, followed by an L-X-Y-C lipoprotein signal peptidase cleavage site. LipL36 is solubilized by Triton X-114 extraction of L. kirschneri; phase separation results in partitioning of LipL36 exclusively into the hydrophobic, detergent phase. LipL36 is intrinsically labeled during incubation of L. kirschneri in media containing [3H]palmitate. Processing of LipL36 is inhibited by globomycin, a selective inhibitor of lipoprotei...Continue Reading

References

Apr 1, 1992·International Journal of Systematic Bacteriology·P RamadassR B Marshall
May 1, 1991·Infection and Immunity·J A BrownM J Pan
Jun 1, 1990·Journal of Bioenergetics and Biomembranes·S Hayashi, H C Wu
Apr 1, 1990·Journal of Clinical Microbiology·T MasuzawaY Yanagihara
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J D RadolfW W Schulz
May 1, 1989·Protein Engineering·G von Heijne
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S MarquseeR L Baldwin
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Marqusee, R L Baldwin
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W I WoodR M Lawn
Jul 1, 1967·Journal of Bacteriology·R C Johnson, V G Harris
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Nov 1, 1995·The Journal of Clinical Investigation·J T SkareM A Lovett
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T G SchwanP A Rosa
Jun 1, 1995·Infection and Immunity·S W BartholdD H Persing
Jul 1, 1995·Infection and Immunity·J D JonesJ D Radolf
Apr 1, 1994·International Journal of Systematic Bacteriology·J L HerrmannG Baranton
Oct 1, 1994·Journal of Bacteriology·D R BlancoM A Lovett
Mar 1, 1993·Microbiological Reviews·A P Pugsley
Nov 15, 1996·Annals of Internal Medicine·J M VinetzD C Kaslow
Apr 1, 1997·Infection and Immunity·K J IndestM T Philipp

❮ Previous
Next ❯

Citations

Dec 19, 2000·Infection and Immunity·J E NallyB Stevenson
Jul 11, 2001·Critical Care Medicine·R J Ulevitch
Apr 12, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Chad J WildNorman E Cheville
Mar 16, 2005·FEMS Microbiology Letters·Marcia GamberiniAna L T O Nascimento
Sep 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Dieter M BulachBen Adler
Aug 4, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C Y FengX K Guo
Sep 22, 2009·Canadian Journal of Microbiology·Wei ZhaoXiao-Kui Guo
Jun 26, 2012·Applied and Environmental Microbiology·Mariko MatsuiCyrille Goarant
Feb 20, 2016·PLoS Neglected Tropical Diseases·Derrick E FoutsJoseph M Vinetz
Apr 6, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A L T O NascimentoE A L Martins
Apr 20, 2002·Current Opinion in Infectious Diseases·J M Vinetz
Jun 4, 2005·Applied and Environmental Microbiology·Sharon L WalkerMenachem Elimelech
Jul 26, 2005·Infection and Immunity·Paul A CullenBen Adler
May 22, 2013·Critical Reviews in Microbiology·Veerapandian Raja, Kalimuthusamy Natarajaseenivasan
Jul 6, 2000·Microbiology·D A Haake
Oct 16, 2002·Infection and Immunity·Raghavan U M PalaniappanHussni O Mohammed
Feb 3, 2005·FEMS Microbiology Letters·Wang-Ju HsiehMing-Jeng Pan
Sep 29, 2004·FEMS Microbiology Reviews·Paul A CullenBen Adler
Apr 16, 2002·Infection and Immunity·Paul A CullenBen Adler
Oct 25, 2007·FEMS Microbiology Letters·Monica L VieiraAna L T O Nascimento
Apr 9, 2001·Clinical Microbiology Reviews·P N Levett
Mar 30, 2011·PloS One·Gustavo M CerqueiraAna L T O Nascimento
Aug 15, 2009·Tropical Animal Health and Production·T M A SenthilkumarV Ramaswamy
Dec 2, 2009·The Journal of Biological Chemistry·Jung-Yu TungYuh-Ju Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cellulitis

Cellulitis (erysipelas) is a recurring and debilitating bacterial infection of the skin and underlying tissue. Discover the latest research on cellulitis here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.