Abstract
In this work we focused on the characterization of a novel plant rennet purified from lettuce leaves (Lactuca sativa L. cv Romana). The lettuce protease, lettucine, showed trypsin-like, SV8-like, and caseinolytic activities. Although the enzyme did not recognize peptides having hydrophobic amino acid residues in the P(1) position of the target bond, it did show milk-clotting activity, suggesting that different bonds rather than the Phe(105)-Met(106) of the kappa-casein might be cleaved, still inducing milk-clotting. The enzyme exhibited proteolytic activity toward alpha-casein, beta-casein, kappa-casein, and milks with different fat contents, with the highest activity observed with partially skimmed milk, total casein, and alpha- and kappa-casein. SDS-PAGE studies showed that lettucine cleaved alpha-casein, beta-casein, and kappa-casein. In particular, we showed that alpha-casein breakdown occurred even though total casein or milks were supplied, suggesting that the lettuce enzyme is able to operate a significant disorganization of the casein's micellar structure. Moreover, the proteolytic activity of the enzyme analyzed under various technological parameters, such as temperature and pH, indicated that the lettuce enzyme is hig...Continue Reading
Citations
Jan 25, 2016·Colloids and Surfaces. B, Biointerfaces·Julia LombardiValeria Boeris
Mar 19, 2016·Food Chemistry·Marilena EspositoRaffaele Porta
Jun 7, 2011·International Journal of Biological Macromolecules·R G BekaD Vercaigne-Marko
Mar 1, 2011·Phytochemistry·Malik Muhammad HashimChen Xiaohong
Jul 27, 2007·Journal of Chromatography. a·Dion M A M LuykxAntoine H P America
Apr 29, 2011·Journal of Dairy Science·X W HuangF Z Ren
Sep 8, 2012·Food Chemistry·Emmanuel V PontualPatrícia M G Paiva
Dec 6, 2014·International Journal of Biological Macromolecules·Mohammed GagaouaKahina Hafid
Feb 13, 2018·Journal of Dairy Science·Jie LuoZhengquan Yu
Jan 23, 2020·Plants·Ivana PuglisiAndrea Baglieri
Mar 27, 2018·Journal of Dairy Science·Z LiY Hemar
Sep 20, 2020·BMC Research Notes·Desta Berhe SbhatuKiros Haddish Tesfamariam
May 21, 2020·Foods·Andrea SerraMarcello Mele
May 31, 2020·Foods·Massimo MozzonLucia Aquilanti
Oct 6, 2018·International Journal of Biological Macromolecules·Moslem AfsharnezhadReyhaneh Sariri
Aug 7, 2021·Heliyon·El-Sayed M M AbdeenAdel M M Kholif