PMID: 11929310Apr 4, 2002Paper

Characterization of "lettucine", a serine-like protease from Lactuca sativa leaves, as a novel enzyme for milk clotting

Journal of Agricultural and Food Chemistry
Angela R Lo PieroGoffredo Petrone

Abstract

In this work we focused on the characterization of a novel plant rennet purified from lettuce leaves (Lactuca sativa L. cv Romana). The lettuce protease, lettucine, showed trypsin-like, SV8-like, and caseinolytic activities. Although the enzyme did not recognize peptides having hydrophobic amino acid residues in the P(1) position of the target bond, it did show milk-clotting activity, suggesting that different bonds rather than the Phe(105)-Met(106) of the kappa-casein might be cleaved, still inducing milk-clotting. The enzyme exhibited proteolytic activity toward alpha-casein, beta-casein, kappa-casein, and milks with different fat contents, with the highest activity observed with partially skimmed milk, total casein, and alpha- and kappa-casein. SDS-PAGE studies showed that lettucine cleaved alpha-casein, beta-casein, and kappa-casein. In particular, we showed that alpha-casein breakdown occurred even though total casein or milks were supplied, suggesting that the lettuce enzyme is able to operate a significant disorganization of the casein's micellar structure. Moreover, the proteolytic activity of the enzyme analyzed under various technological parameters, such as temperature and pH, indicated that the lettuce enzyme is hig...Continue Reading

References

Mar 1, 1996·Applied Biochemistry and Biotechnology·T Uchikoba, M Kaneda

❮ Previous
Next ❯

Citations

Jun 7, 2011·International Journal of Biological Macromolecules·R G BekaD Vercaigne-Marko
Mar 1, 2011·Phytochemistry·Malik Muhammad HashimChen Xiaohong
Apr 29, 2011·Journal of Dairy Science·X W HuangF Z Ren
Sep 8, 2012·Food Chemistry·Emmanuel V PontualPatrícia M G Paiva
Dec 6, 2014·International Journal of Biological Macromolecules·Mohammed GagaouaKahina Hafid
Sep 20, 2020·BMC Research Notes·Desta Berhe SbhatuKiros Haddish Tesfamariam
Oct 6, 2018·International Journal of Biological Macromolecules·Moslem AfsharnezhadReyhaneh Sariri
Aug 7, 2021·Heliyon·El-Sayed M M AbdeenAdel M M Kholif

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.