Characterization of LF9, an octoploid strawberry genotype selected for rapid regeneration and transformation.

Planta
Kevin M FoltaCraig K Chandler

Abstract

Cultivated strawberry (Fragaria xananassa) is a valuable crop, yet the absence of a rapid, high-throughput transgenic system has precluded meaningful application of biotechnology and translation of information from plant models to this crop. A new octoploid strawberry genetic line Laboratory Festival #9 has been identified, selected solely for its rapid regeneration and efficient transformation. Direct organogenesis has been achieved from all tissues tested, with rapidly-growing shoot initials visible in as few as 13 days. The conditions for optimal shoot regeneration, transformant selection, root generation, and plant acclimatization are presented. The progression from explant to plant in soil can be achieved in about 60 days. The development of transformation protocols in this rapid-cycling genotype allows high-throughput studies of gene function in the octoploid strawberry genetic background.

References

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Citations

Dec 18, 2013·Journal of Agricultural and Food Chemistry·Alan H ChambersKevin M Folta
Feb 21, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sara SharifiHamidreza Ghasempour
Feb 19, 2008·Biotechnology Advances·Yonghua QinShanglong Zhang
Jul 1, 2015·Frontiers in Plant Science·Michela Guidarelli, Elena Baraldi
Dec 11, 2013·GM Crops & Food·Hossam ZakariaNaglaa A Abdallah
Feb 26, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fatemeh HaddadiHossein Kamaladini

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