Characterization of lipid A Cronobacter sakazakii

European Journal of Mass Spectrometry
Chan ZhangX Wang

Abstract

We have characterized the structure of lipid A in food-borne pathogen Cronobacter sakazakii BAA-894. Lipid A molecules were purified by mild acid hydrolysis, DEAE-cellulose anion exchange chromatography and preparative thin-layer chromatography. Its structure was analyzed by electrospray ionization/mass spectrometry. Two lipid A species have been found in C. sakazakii BAA-894. The majority has a structure of disaccharide of glucosamine, phosphorylated at 1- and 4'-positions, and acylated with primary 3-hydroxymyristoyl chains at 2-, 3-, 2'- and 3'-positions and secondary myristoyl residues at 2'- and 3'- positions. The other contains a secondary lauroyl chain at 2'-position in place of the myristoyl residue.

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Citations

May 20, 2014·Biological Reviews of the Cambridge Philosophical Society·Xiaoyuan WangAixin Yan
Nov 19, 2015·European Journal of Mass Spectrometry·Yuqian WangXiaoyuan Wang
Jan 18, 2018·Scientific Reports·Seongok KimSangryeol Ryu
Aug 30, 2018·Frontiers in Microbiology·Si ChenXiaoyuan Wang
Aug 10, 2016·Rapid Communications in Mass Spectrometry : RCM·Yanyan LiDavid R Goodlett

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