Abstract
Lipopolysaccharide is a major constituent of the cell surface of the gram-negative procaryote Myxococcus xanthus. We have purified lipopolysaccharide from M. xanthus and have shown by silver staining that the lipopolysaccharide contains a heterogeneous population of molecules which migrate as a broad low-molecular-mass band (approximately 5 kilodaltons) and as a stepladder of about 30 higher-molecular-mass bands (15- to 70-kilodalton range). The broad band consists of lipopolysaccharide molecules with just lipid A and core regions. The stepladder bands contain lipopolysaccharide molecules with lipid A, core regions, and various numbers of O-antigen units. Monoclonal antibodies generated against the cell surface of developing M. xanthus cells (J. S. Gill and M. Dworkin, Proc. Natl. Acad. Sci. USA 84:4505-4508, 1987) were used to help characterize the lipopolysaccharide molecules. Five monoclonal antibodies bound to carbohydrate epitopes on the stepladder but not to the broad band, indicating that these monoclonal antibodies recognize carbohydrates on the O antigen of the lipopolysaccharide molecules. Four of these five monoclonal antibodies bound to doublet bands in the stepladder, while the other monoclonal antibody bound to si...Continue Reading
References
Dec 1, 1979·Infection and Immunity·M A Apicella, N C Gagliardi
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1975·Journal of General Microbiology·I W Sutherland, S Thomson
Jan 1, 1976·Nature·I W Sutherland
Nov 1, 1986·Journal of Bacteriology·J S Gill, M Dworkin
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J S GillM Dworkin
Nov 1, 1985·Journal of Bacteriology·S M Panasenko
May 15, 1974·European Journal of Biochemistry·G RosenfelderO Westphal
Mar 1, 1969·Journal of Bacteriology·M NiwaJ A Rudbach
Dec 22, 1971·European Journal of Biochemistry·J WeckesserO Lüderitz
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1983·Journal of Bacteriology·P J Hitchcock, T M Brown
Aug 1, 1983·Analytical Biochemistry·K Hancock, V C Tsang
Apr 1, 1984·The Journal of Infectious Diseases·C S MintzS A Morse
Jan 1, 1984·Analytical Biochemistry·M S BlakeE C Gotschlich
Feb 1, 1980·Journal of Bacteriology·P E Orndorff, M Dworkin
Jan 1, 1980·European Journal of Biochemistry·E T Palva, P H Mäkelä
Aug 1, 1962·Journal of Bacteriology·M DWORKIN
Citations
Sep 1, 1989·Molecular Microbiology·L J Shimkets
Nov 5, 1999·Annual Review of Microbiology·L J Shimkets
Sep 9, 2000·FEMS Microbiology Reviews·W Dawid
Aug 12, 2020·Journal of Bacteriology·María Pérez-BurgosLotte Søgaard-Andersen
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andrew E PellingJames K Gimzewski
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yinuo LiWenyuan Shi
Oct 29, 1998·Molecular Microbiology·M G Bowden, H B Kaplan
Jul 25, 2019·Molecular Microbiology·María Pérez-BurgosLotte Søgaard-Andersen
Oct 10, 1998·Journal of Bacteriology·R M WeimerP L Hartzell
Aug 19, 2003·Journal of Bacteriology·Vesna SimunovicLawrence J Shimkets
Sep 1, 1989·Journal of Bacteriology·B W Jarvis, M Dworkin
Apr 1, 1989·Journal of Bacteriology·J M Fink, J F Zissler
Sep 1, 1989·Journal of Bacteriology·B W Jarvis, M Dworkin
Apr 1, 1989·Journal of Bacteriology·J M FinkJ F Zissler
May 1, 1992·Journal of Bacteriology·M I Ramos-GonzálezJ L Ramos
Mar 1, 1996·Journal of Bacteriology·D GuoH B Kaplan
Sep 1, 1992·Journal of Bacteriology·B Y Chang, D White
Nov 1, 1990·Journal of Bacteriology·M Kalos, J F Zissler
Nov 1, 1991·Journal of Bacteriology·D L ClemansM Dworkin
Dec 1, 1990·Microbiological Reviews·L J Shimkets