PMID: 2495269Apr 1, 1989Paper

Characterization of lipopolysaccharide from Myxococcus xanthus by use of monoclonal antibodies

Journal of Bacteriology
J M Fink, J F Zissler

Abstract

Lipopolysaccharide is a major constituent of the cell surface of the gram-negative procaryote Myxococcus xanthus. We have purified lipopolysaccharide from M. xanthus and have shown by silver staining that the lipopolysaccharide contains a heterogeneous population of molecules which migrate as a broad low-molecular-mass band (approximately 5 kilodaltons) and as a stepladder of about 30 higher-molecular-mass bands (15- to 70-kilodalton range). The broad band consists of lipopolysaccharide molecules with just lipid A and core regions. The stepladder bands contain lipopolysaccharide molecules with lipid A, core regions, and various numbers of O-antigen units. Monoclonal antibodies generated against the cell surface of developing M. xanthus cells (J. S. Gill and M. Dworkin, Proc. Natl. Acad. Sci. USA 84:4505-4508, 1987) were used to help characterize the lipopolysaccharide molecules. Five monoclonal antibodies bound to carbohydrate epitopes on the stepladder but not to the broad band, indicating that these monoclonal antibodies recognize carbohydrates on the O antigen of the lipopolysaccharide molecules. Four of these five monoclonal antibodies bound to doublet bands in the stepladder, while the other monoclonal antibody bound to si...Continue Reading

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Citations

Nov 5, 1999·Annual Review of Microbiology·L J Shimkets
Sep 9, 2000·FEMS Microbiology Reviews·W Dawid
Aug 12, 2020·Journal of Bacteriology·María Pérez-BurgosLotte Søgaard-Andersen
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andrew E PellingJames K Gimzewski
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yinuo LiWenyuan Shi
Aug 19, 2003·Journal of Bacteriology·Vesna SimunovicLawrence J Shimkets
Sep 1, 1989·Journal of Bacteriology·B W Jarvis, M Dworkin
Apr 1, 1989·Journal of Bacteriology·J M Fink, J F Zissler
Sep 1, 1989·Journal of Bacteriology·B W Jarvis, M Dworkin
Nov 1, 1990·Journal of Bacteriology·M Kalos, J F Zissler
Dec 1, 1990·Microbiological Reviews·L J Shimkets

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