Characterization of Listeria monocytogenes isolates of food and human origins from Brazil using molecular typing procedures and in vitro cell culture assays

International Journal of Environmental Health Research
Valter F BuenoArun K Bhunia

Abstract

The spreading of diseases through foods is a worldwide concern. Here, molecular and in vitro cell-culture assays were employed to characterize 63 Brazilian Listeria monocytogenes isolates (food, 47; clinical, 16). Serotype 4b was the most predominant (49%) followed by (1/2)b (30%), (1/2)a (10%), (1/2)c (6%), 3c (3%) and 3b (2%). Ribotyping yielded 17 ribopatterns, which were grouped into four phylogenetic clusters. Cluster A comprised of 39/63 isolates primarily of food origin, and clusters B, C and D contained both food and clinical isolates. Isolates were positive for virulence determinants prfA, hlyA and inlA: clinical isolates were more invasive to Caco-2 cells and expressed high levels of inlA transcripts than the food isolates. Highly invasive isolates also provoked more Ped-2E9 cells to die by apoptosis than the weakly-invasive strains. These data demonstrate a strong genetic relatedness among clinical and food isolates and suggest transmission of a subset of L. monocytogenes strains from food to humans.

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Citations

Mar 11, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Anderson Carlos CamargoLuís Augusto Nero
Apr 20, 2017·The Journal of Veterinary Medical Science·Rie HasebeMotohiro Horiuchi
May 30, 2019·Frontiers in Microbiology·Xiaoai ZhangXiaochen Ma

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Methods Mentioned

BETA
ribotyping
PCR
Fluorescence microscopy
Fluorescence

Software Mentioned

UPGMA
SAS
SPOT
Ribotyping
Bionumerics
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis