Characterization of liver-cirrhosis nodules by analysis of gene-expression profiles and patterns of allelic loss

Journal of Human Genetics
H NagaiM Emi

Abstract

To disclose genetic mechanisms involved in development or progression of hepatocellular carcinoma (HCC), we used a genome-wide cDNA microarray consisting of 8,448 genes to compare gene-expression profiles among 12 liver-cirrhosis nodules (LCNs) and five specimens of HCC excised from a single patient and carefully prepared by laser-capture microdissection (LCM). The expression patterns enabled us to identify 72 genes that were frequently upregulated and 57 that were downregulated specifically in the LCN specimens as compared to the HCCs. We also documented upregulation of 31 genes and downregulation of seven others in both HCC and LCN tissues. Several types of intracellular kinase, including receptor-type kinase, were upregulated in LCNs. Expression patterns of HCCs and LCNs generally represented two genetically distinct groups when subjected to a hierarchical clustering analysis, although expression profiles of two of the LCNs resembled the HCC pattern. Analysis of allelic losses at microsatellite loci revealed that LCNs showed frequent loss of heterozygosity (LOH) (33%) in chromosomal regions 6q and 22q; over half of the LCNs had lost an allele for at least one of the 28 loci examined. The presence of early genetic changes amo...Continue Reading

References

Apr 11, 1986·Nucleic Acids Research·K KawakamiK Nagano
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·T YamadaR L Jirtle
Dec 31, 1997·Science·R F BonnerL A Liotta
Aug 4, 1998·The EMBO Journal·R C Patel, G C Sen
Feb 2, 1999·Oncogene·M SalvucciB Debuire
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·K LevayV Z Slepak
Mar 25, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M RoncalliG Coggi

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Citations

Oct 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Pierre J ZindyNathalie Théret
Dec 24, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yutaka MidorikawaHiroyuki Aburatani
Jul 13, 2005·Biochemical and Biophysical Research Communications·Soyoun Kim, Young Min Park
Oct 12, 2007·Journal of Biochemistry and Molecular Biology·Seram Lee, Soyoun Kim
Sep 19, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Kathy Y-Y ChanNathalie Wong
Sep 7, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Joseph Locker

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