PMID: 9524713Apr 3, 1998Paper

Characterization of local inflammatory response in an isolated lung perfusion model

Annals of Surgical Oncology
A AbolhodaM Burt

Abstract

Current phase I trials of isolated lung perfusion for treatment of pulmonary metastases have an arbitrarily determined length of perfusion. Our objective was to examine the temporal course of the local and distant inflammatory response as a function of the length of perfusion (ischemia) and subsequent reperfusion in an equivalent animal model. Sixty male Fischer 344 rats were randomized into four groups (n = 15). Each group underwent left isolated lung perfusion with buffered Hespan for 10, 30, 60, or 90 minutes. Subsequently, two subgroups of five animals within each group were allowed to reperfuse for 1 or 3 hours, respectively. Non-perfused right lung was used as control. At each time point, lung specimens were assayed for TNF-alpha by ELISA and histologic sections were examined. There was no significant difference between the left and right lung tissue levels of TNF-alpha at the termination of the ischemic period. However, on reperfusion, the left lung TNF-alpha levels increased significantly above the ischemia baseline in all groups, with a greater magnitude of rise in the groups with 60 and 90 minutes of preceding ischemia (12,757 +/- 1985 vs. 3524 +/- 494 pg/g, and 16,914 +/- 1657 vs. 6530 +/- 1104 pg/g, respectively; p ...Continue Reading

References

Mar 1, 1979·The Annals of Thoracic Surgery·D L Modry, R C Chiu
Apr 1, 1992·Journal of Applied Physiology·R G EckenhoffA B Fisher
Mar 1, 1990·The American Review of Respiratory Disease·J Kelley
Dec 1, 1990·The Journal of Clinical Investigation·T J StandifordR M Strieter
Jan 1, 1990·Journal of Applied Physiology·T F KowalskiM Bishop
Aug 1, 1983·Cancer·M R JohnstonM R Boyd
Jun 1, 1994·The Annals of Thoracic Surgery·H W PogrebniakH I Pass
Jan 1, 1994·Journal of Applied Physiology·D B Pearse, E M Wagner
Jun 1, 1993·Journal of Applied Physiology·B WekslerM Burt
Jan 1, 1993·The American Review of Respiratory Disease·G P PalaceA B Malik
Feb 1, 1993·The American Review of Respiratory Disease·M SuW R Henderson
Jun 1, 1996·The Annals of Thoracic Surgery·H I PassS A Rosenberg
Jul 1, 1996·The Annals of Thoracic Surgery·R J NovickJ Lee
Oct 1, 1994·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·S Kanwar, P Kubes
Sep 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·G B RattoG Fantino

❮ Previous
Next ❯

Citations

Dec 8, 1998·Journal of Applied Physiology·A B Al-Mehdi, A B Fisher
Jun 30, 2006·Thoracic Surgery Clinics·Jeroen M H HendriksPaul E Y Van Schil

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.