Characterization of long non-coding RNAs and MEF2C-AS1 identified as a novel biomarker in diffuse gastric cancer

Translational Oncology
Tianhang LuoXuchao Xue

Abstract

Previous studies proved that long noncoding RNAs (lncRNAs) play important role in human cancer. However, the knowledge of genome scale expression of lncRNAs and their potential biological function in gastric cancer is still lacking. Next generation RNA sequencing (RNA-seq) was performed on tumor tissues and matched adjacent normal tissues of six diffuse gastric cancer (DGC) patients. Then we performed a comprehensive analysis on lncRNAs and mRNA. Fifty-eight lncRNAs were upregulated and 54 lncRNAs were downregulated in diffuse gastric cancer tissue compared with adjacent tissue. The numbers of up- and downregulated mRNAs were 306 and 161, respectively. In addition, we inferred the function of lncRNAs by construction of a co-expression network for deregulated mRNAs and lncRNAs. Co-expressed genes of MEF2C-AS1 and FENDRR were enriched to RAS and TGF-beta signaling pathway. MEF2C-AS1 and FENDRR expression were re-evaluated by Real-time Quantitative PCR in 42 DGC patients' tumor and normal tissues, and other 46 DGC patents' and 21 healthy controls' plasma. Validation data showed MEF2C-AS1 and FENDRR were significantly downregulated in tumor tissues compared with normal tissues. And decreased FENDRR are associated with aggressive tu...Continue Reading

Citations

Mar 4, 2020·Molecular and Cellular Biochemistry·Isabela Tiemy PereiraBruno Dallagiovanna
Jan 4, 2020·Experimental and Molecular Pathology·Soudeh Ghafouri-Fard, Mohammad Taheri
Dec 6, 2020·Journal of Hematology & Oncology·Mingye HongHua Zhang
Dec 29, 2020·Technology in Cancer Research & Treatment·Chuan ChengJianping Wang
Mar 26, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qingyuan ZhengWenzhi Guo
Aug 24, 2021·Frontiers in Molecular Biosciences·Qingshui WangYao Lin

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Methods Mentioned

BETA
RNA-seq
PCR
ELISA
RNAS-seq

Software Mentioned

SPSS
DESeq2
Cytoscap
HISAT2
R
GENCODE
Ensembl
GraphPad Prism
ClusterProfiler
StringTie

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