Characterization of low-acetic-acid-producing yeast isolated from 2-deoxyglucose-resistant mutants and its application to high-gravity brewing

Journal of Bioscience and Bioengineering
Akihiro MizunoMasahumi Iwahuti

Abstract

We isolated a mutant with low acetic acid and high ethanol productivities from 2-deoxyglucose-resistant mutants of brewers' yeast NCYC1245 (Saccharomyces cerevisiae). To determine the mechanism for these properties in the mutant (2DGR19) during fermentation, gene expression and enzyme activity related to acetic acid and ethanol production were investigated. DNA microarray analysis revealed that the transcriptional levels of many genes involved in glycolysis were higher in 2DGR19 than in NCYC1245. Among these transcriptional levels of 2DGR19 relative to NCYC1245, the expression level of ADH4 encoding alcohol dehydrogenase (ADH) was highest, which corresponded to the high ADH activity in 2DGR19. Quantitative PCR analysis also revealed that the transcriptional level of ADH4 was the highest among ADH1 to ADH4. Although no significant differences in the transcriptional levels of ALD2 to ALD6 encoding acetaldehyde dehydrogenase (ALD) between 2DGR19 and NCYC1245 were observed, ALD activity in 2DGR19 was lower. Using quantitative PCR analysis, ALD6 was found to be the most highly expressed among the ALD2 to ALD6 genes. These results indicate that ALD6 contributes to a low ALD activity, depending on post-transcriptional regulation. A hi...Continue Reading

Citations

Jun 23, 2011·Journal of Industrial Microbiology & Biotechnology·Pradeep PuligundlaSanghoon Ko
Mar 3, 2010·Applied Microbiology and Biotechnology·Sofie M G SaerensElke Nevoigt
May 16, 2008·FEMS Yeast Research·Olga de SmidtJacobus Albertyn
Nov 14, 2012·FEMS Yeast Research·Antonio G CordenteChristopher D Curtin
Oct 24, 2020·The Journal of Microbiology·Xi ZhangYu Deng
Dec 2, 2006·Journal of Proteome Research·Trong Khoa PhamPhillip C Wright

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