Characterization of lycopene-overproducing E. coli strains in high cell density fermentations

Applied Microbiology and Biotechnology
Hal AlperG Stephanopoulos

Abstract

Previous work identified two recombinant strains of Escherichia coli capable of significant lycopene overproduction. These strains were constructed by superimposing the deletion of three genes, selected through combinatorial and systematic searches of the metabolic landscape, onto a previously engineered strain over-expressing critical genes in the lycopene biosynthesis pathway. In this paper, we characterize the performance of these two strains in comparison to the parental, pre-engineered strain. Specifically, high cell density fermentations were performed after identifying optimized putative operating parameters. High oxygen levels and increased pH values were found to be critical for increasing both specific and volumetric product titers. Carbon balances suggest linkages between glutamate, NADPH, formate, and alanine levels with lycopene overproduction. Furthermore, lycopene production reached nearly 220 mg/l from approximately 27 g dry cell weight/l in these reactors, which is the highest value reported to date for E. coli.

References

Aug 1, 1991·Journal of Biotechnology·D RiesenbergW D Deckwer
May 29, 1992·Biochemical and Biophysical Research Communications·P D Fraser, G Sandmann
Sep 27, 2002·Chembiochem : a European Journal of Chemical Biology·Gerhard Sandmann
Oct 17, 2002·Applied Microbiology and Biotechnology·P C Lee, C Schmidt-Dannert
Jun 5, 2003·Nature Biotechnology·Vincent J J MartinJay D Keasling

❮ Previous
Next ❯

Citations

Oct 4, 2007·Applied Microbiology and Biotechnology·Amitabha DasSeon-Won Kim
Feb 2, 2008·Applied Microbiology and Biotechnology·Hal Alper, Gregory Stephanopoulos
Aug 19, 2011·Applied Microbiology and Biotechnology·Brett A BoghigianBlaine A Pfeifer
Sep 3, 2013·Applied Biochemistry and Biotechnology·Qiang WangXiao-Bin Yu
Mar 30, 2010·Applied and Environmental Microbiology·Hyung Seok ChoiHan Min Woo
May 9, 2014·Biotechnology Letters·Tao SunYanhe Ma
Apr 2, 2014·Applied Microbiology and Biotechnology·Sabine A E HeiderTrygve Brautaset
Jul 7, 2009·Annual Review of Plant Biology·James Kirby, Jay D Keasling
May 8, 2010·Current Opinion in Microbiology·Vikramaditya G Yadav, Gregory Stephanopoulos
Nov 11, 2008·Current Opinion in Biotechnology·Joseph A Chemler, Mattheos A G Koffas
Jan 24, 2007·Metabolic Engineering·Douglas J PiteraJay D Keasling
Jan 20, 2010·Biotechnology Journal·Pamela P Peralta-Yahya, Jay D Keasling
Feb 13, 2010·Biotechnology Journal·John Blazeck, Hal Alper
Jul 19, 2015·Journal of Industrial Microbiology & Biotechnology·Irene MartínezAlvaro Díaz-Barrera
May 19, 2007·Metabolic Engineering·Yong-Su Jin, Gregory Stephanopoulos
Nov 27, 2015·Microbiology and Molecular Biology Reviews : MMBR·Paulo MaiaIsabel Rocha
Jun 24, 2014·Molecular Systems Biology·Iman FarasatHoward M Salis
Jul 30, 2014·Natural Product Reports·Myoung Chong SongYeo Joon Yoon
May 19, 2018·Frontiers in Microbiology·April M MiguezMark P Styczynski
Jul 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhaobao WangJianming Yang
May 30, 2020·Biotechnology Advances·Gabriel Capson-TojoTim Hülsen
Feb 1, 2008·Molecular Pharmaceutics·Haoran ZhangBlaine A Pfeifer
Mar 22, 2008·Molecular Pharmaceutics·Parayil Kumaran AjikumarGregory Stephanopoulos
Jun 1, 2018·Journal of Agricultural and Food Chemistry·Parise AdadiElena Fedorovna Krivoshapkina
Sep 19, 2019·Journal of Agricultural and Food Chemistry·Bin ShiTiangang Liu
Aug 23, 2021·Molecular Biotechnology·Dipinte GuptaRajiv Ranjan
May 29, 2018·Journal of Agricultural and Food Chemistry·Anping SuYonghong Meng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.