Characterization of lympho-myeloid-erythroid-megakaryocytic stem cells in peripheral blood of hairy cell leukemia patients.

Leukemia Research
R MichaleviczE Sahar

Abstract

Circulating stem cells with lympho-myeloid-erythroid differentiative capacity have been described in the peripheral blood of hairy cell leukemia (HCL) patients (Michalevicz R. & Revel M. (1987) Interferons regulate the in vitro differentiation of multilineage lympho-myeloid stem cells in Hairy Cell Leukemia. Proc. natn. Acad. Sci. U.S.A. 84, 2307.) The aim of the present work was to enrich the progenitors and characterize their antigenic and growth properties. Peripheral blood (PB) from HCL patients was stained with antibodies (BI3C5 (CD34) and/or My10 as well as RFB7 (CD20) and RFT12 (CD7) and sorted using flow cytometry (FCM) into positive and negative fractions. Peripheral blood cells from several patients showed 4-16% cells positive for the BI3C5/My10. The positive fraction contained all the colony-forming cells and LGEM/LG/LGM colonies were enriched approximately ten-fold as compared to the unsorted population. No colony was found in the negative fraction. All colony forming cells were in the RFB7 and RFT12-negative fractions. Thus, circulating stem cells with lymphoid/myeloid potential can be isolated from PB of HCL patients.

References

Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R Michalevicz, M Revel
Sep 1, 1988·Medical Hypotheses·R Michalevicz, I Nada
Jul 1, 1986·British Journal of Haematology·R MichaleviczA V Hoffbrand
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A A FauserG W Löhr
Mar 1, 1985·The Journal of Clinical Investigation·A A FauserG W Löhr
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S J KorsmeyerT A Waldmann

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Citations

Oct 1, 1991·British Journal of Haematology·H E HeslopA V Hoffbrand
May 1, 1995·British Journal of Haematology·M SchmidF Porzsolt
Oct 27, 2018·Journal of Cellular Physiology·Fatemeh VahidianBehzad Baradaran

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