Characterization of lymphocyte subsets in ascitic fluid and peripheral blood of decompensated cirrhotic patients with chronic hepatitis C and alcoholic liver disease: A pivotal study.

International Journal of Immunopathology and Pharmacology
Roberto Giulio RomanelliFabio Almerigogna

Abstract

Hepatitis C virus and alcoholic liver disease are major causes of chronic liver diseases worldwide. Little is known about differences between chronic hepatitis C and alcoholic liver disease in terms of lymphocytes' sub-population. Aim of the present study was to compare the sub-populations of lymphocytes in both ascitic compartment and peripheral blood in patients with decompensated liver cirrhosis due to chronic hepatitis C and alcoholic liver disease. Patients with decompensated liver cirrhosis due to hepatitis C virus or alcoholic liver disease evaluated from April 2014 to October 2016 were enrolled. Whole blood and ascitic fluid samples were stained with monoclonal antibodies specific for human TCRɑβ, TCRɣδ, CD3, CD4, CD8, CD19, CCR6, CD16, CD56, CD25, HLA-DR, Vɑ24. Sixteen patients with decompensated liver cirrhosis were recruited (9 with hepatitis C virus and 7 with alcoholic liver disease). In ascitic fluid, the percentage of both CD3+CD56- and CD3+CD56+iNKT cells resulted higher in hepatitis C virus patients than in alcoholic liver disease patients (1.82 ± 0.35% vs 0.70 ± 0.42% (p < 0.001) and 1.42 ± 0.35% vs 0.50 ± 0.30% (p < 0.001), respectively). Conversely, in peripheral blood samples, both CD3+CD56- and CD3+CD56+iN...Continue Reading

References

Mar 27, 2003·Nature Reviews. Immunology·S Brian Wilson, Terry L Delovitch
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudia de LallaPaolo Dellabona
Jan 11, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Hans J J van der VlietCarin J M van Nieuwkerk
May 25, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Maya Margalit, Yaron Ilan
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Markus Biburger, Gisa Tiegs
Oct 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shinichiro MotohashiToshinori Nakayama
Jan 26, 2007·Alcoholism, Clinical and Experimental Research·Chiung M ChenDiane L Lucas
Mar 6, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Matthew WiseFrank Sorvillo
Jul 8, 2008·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·S GittoM Bernardi
Oct 2, 2012·Biochimica Et Biophysica Acta·Bin Gao, Svetlana Radaeva
May 17, 2014·World Journal of Gastroenterology : WJG·Salman NusratMohammad F Madhoun
Oct 30, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Abhisek MitraShulin Li
Dec 6, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Igor MaricicVipin Kumar
Dec 22, 2014·The Journal of Allergy and Clinical Immunology·Francesco AnnunziatoSergio Romagnani
May 6, 2015·Journal of Clinical and Experimental Hepatology·Richard MoreauVicente Arroyo
Jan 29, 2016·World Journal of Gastroenterology : WJG·Joo Chun YoonJae Myun Lee
Mar 15, 2016·Cellular & Molecular Immunology·Keya BandyopadhyayVipin Kumar
Jun 15, 2016·Current Drug Targets·Roberto Giulio Romanelli, Cristina Stasi
Jul 4, 2018·Nature Reviews. Immunology·Catherine M Crosby, Mitchell Kronenberg
Sep 27, 2018·Frontiers in Immunology·Idania MarreroVipin Kumar

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy

Software Mentioned

FACSDiva

Related Concepts