Characterization of lymphocytes in the adult rat testis by flow cytometry: effects of activin and transforming growth factor beta on lymphocyte subsets in vitro

Biology of Reproduction
A B TompkinsM P Hedger

Abstract

The rat testis is considered to be an immunologically privileged site because of its reduced capacity to support antigen-specific immune responses. To understand this phenomenon, it is essential to characterize both the lymphocyte subpopulations normally present in the testis and their regulation by testicular cytokines. Peripheral blood was obtained from adult male Dark Agouti or Sprague-Dawley rats, and testicular interstitial tissue was collected after perfusion of the testes to remove blood. Blood and testis lymphocytes were isolated using discontinuous Percoll density gradients, and the testicular lymphocytes were further purified by selective adherence to remove mononuclear phagocytes. The isolated lymphocytes were analyzed by flow cytometry using specific monoclonal antibodies and fluorescein labeling and were enumerated as total T cells, CD4+ T cells, CD8+ T cells, B cells, and natural killer (NK) cells. In contrast to peripheral blood, in which the CD4+ T-cell subset was the major lymphocyte subset, rat testis T cells were predominantly of the CD8+ subset, and a large population of NK cells also were present. Subsequently, peripheral blood lymphocytes were stimulated with the polyclonal T-cell activator, phytohemagglut...Continue Reading

Citations

Oct 19, 2002·Journal of Reproductive Immunology·Mark P Hedger
May 4, 2011·Immunology and Cell Biology·G R Aleman-Muench, G Soldevila
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Jul 4, 2021·Reproduction in Domestic Animals = Zuchthygiene·Qian ZhangZhanchun Bai
Aug 10, 2021·Frontiers in Immunology·Lijun YeLianghui Diao
Apr 7, 2010·Molecular and Cellular Endocrinology·Andreas Meinhardt, Mark P Hedger

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