PMID: 2121880Nov 1, 1990Paper

Characterization of macrophage subsets regulating murine natural killer cell activity

Journal of Leukocyte Biology
J A NelsonP K Lala

Abstract

We examined the relationship of I-A expression by normal murine macrophages to their immunoregulatory role on natural killer cell activity. Macrophages were isolated on the basis of plastic adherence; characterized on the basis of conventional markers such as phagocytic ability, cytoplasmic non-specific esterase activity, surface MAC-1 and F4/80 antigen expression; and then used for functional studies relative to their expression of surface I-A. Two functional macrophage subsets were identified: NK-stimulatory and NK-suppressive subsets. The former function was associated with splenic macrophages, which were predominantly I-A+ as identified with a radioautographic immunolabeling technique; the latter function with peritoneal macrophages which were predominantly I-A-. Loss of macrophage I-A expression in vitro was delayed in the presence of indomethacin and enhanced in the presence of PGE2, indicating that PGE2 down-regulates I-A expression on macrophages. The NK stimulatory function of I-A+ macrophages was attributable to a soluble mediator, identified as IFN-gamma, since the stimulatory ability was abrogated with an anti-IFN-gamma antibody. I-A expression appears to be important for the stimulatory function, since some interfe...Continue Reading

Citations

Jul 15, 1998·International Journal of Cancer. Journal International Du Cancer·C SchmidtP De Baetselier
Aug 1, 1989·American Journal of Reproductive Immunology : AJRI·P K Lala
May 1, 1993·Clinical & Experimental Metastasis·M N SaarloosP K Lala
Sep 18, 1997·Journal of Applied Physiology·S E BlankG G Meadows
Sep 14, 1994·Journal of Immunological Methods·N Plasman, B Vray

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.